Goverman J, Gomez S M, Segesman K D, Hunkapiller T, Laug W E, Hood L
Division of Biology, California Institute of Technology, Pasadena 91125.
Cell. 1990 Mar 23;60(6):929-39. doi: 10.1016/0092-8674(90)90341-b.
We constructed chimeric receptor chains in which an immunoglobulin heavy chain variable region (VH) from a phosphorylcholine-specific antibody is substituted for T cell receptor (Tcr) alpha and beta V regions. We demonstrate that the VH region joined to either the C alpha or the C beta region can form stable chimeric proteins in EL4 T cells. Both chimeric receptor chains associate with CD3 polypeptides in functional receptor complexes and respond to phosphorylcholine coupled to Sepharose beads. The VH-C alpha chimeric chain associates with the EL4 beta chain, while the VH-C beta chimeric protein appears to form either a homodimer or a heterodimer with the native EL4 beta chain. Thus, functional receptor complexes can be formed using two C beta regions, and the C alpha region may not be required for CD3 association and surface expression of Tcr complexes.
我们构建了嵌合受体链，其中来自磷酸胆碱特异性抗体的免疫球蛋白重链可变区（VH）取代了T细胞受体（Tcr）的α和β可变区。我们证明，与Cα或Cβ区域连接的VH区域可在EL4 T细胞中形成稳定的嵌合蛋白。两种嵌合受体链都在功能性受体复合物中与CD3多肽结合，并对偶联到琼脂糖珠上的磷酸胆碱产生反应。VH-Cα嵌合链与EL4β链结合，而VH-Cβ嵌合蛋白似乎与天然EL4β链形成同二聚体或异二聚体。因此，使用两个Cβ区域可以形成功能性受体复合物，并且CD3结合和Tcr复合物的表面表达可能不需要Cα区域。
