负责与CD3相互作用的T细胞受体α链和β链区域。

Regions of the T cell receptor alpha and beta chains that are responsible for interactions with CD3.

作者信息

Tan L, Turner J, Weiss A

机构信息

Howard Hughes Medical Institute, Department of Medicine, San Francisco, California.

出版信息

J Exp Med. 1991 May 1;173(5):1247-56. doi: 10.1084/jem.173.5.1247.

DOI:10.1084/jem.173.5.1247

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2118847/

Abstract

The T cell antigen receptor consists of the Ti alpha/beta heterodimer which recognizes antigen, and the associated CD3 chains, thought to be involved in signal transduction. To understand the nature of the interaction between Ti and CD3, chimeric molecules which included the COOH-terminal segments of Ti alpha or beta linked to the extracellular segment of CD8, were transfected into a mutant T cell deficient in Ti beta chain expression and cell surface CD3. Both chimeric chains were required to express the chimeric Ti and to restore CD3 surface expression. CD8/Ti and CD3 cointernalized and coimmunoprecipitated. Stimulation of the chimeric receptor induced transmembrane signaling events and cell activation. These results demonstrate that the Ti alpha and beta COOH termini containing the transmembrane domains are sufficient for structural and functional coupling of Ti to CD3.

摘要

T细胞抗原受体由识别抗原的Tiα/β异二聚体和被认为参与信号转导的相关CD3链组成。为了了解Ti与CD3之间相互作用的本质，将包含与CD8细胞外段相连的Tiα或β的COOH末端片段的嵌合分子转染到缺乏Tiβ链表达和细胞表面CD3的突变T细胞中。表达嵌合Ti和恢复CD3表面表达都需要两条嵌合链。CD8/Ti和CD3共同内化并共同免疫沉淀。嵌合受体的刺激诱导跨膜信号转导事件和细胞活化。这些结果表明，包含跨膜结构域的Tiα和β的COOH末端足以实现Ti与CD3的结构和功能偶联。

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