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负责与CD3相互作用的T细胞受体α链和β链区域。

Regions of the T cell receptor alpha and beta chains that are responsible for interactions with CD3.

作者信息

Tan L, Turner J, Weiss A

机构信息

Howard Hughes Medical Institute, Department of Medicine, San Francisco, California.

出版信息

J Exp Med. 1991 May 1;173(5):1247-56. doi: 10.1084/jem.173.5.1247.

DOI:10.1084/jem.173.5.1247
PMID:1827143
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2118847/
Abstract

The T cell antigen receptor consists of the Ti alpha/beta heterodimer which recognizes antigen, and the associated CD3 chains, thought to be involved in signal transduction. To understand the nature of the interaction between Ti and CD3, chimeric molecules which included the COOH-terminal segments of Ti alpha or beta linked to the extracellular segment of CD8, were transfected into a mutant T cell deficient in Ti beta chain expression and cell surface CD3. Both chimeric chains were required to express the chimeric Ti and to restore CD3 surface expression. CD8/Ti and CD3 cointernalized and coimmunoprecipitated. Stimulation of the chimeric receptor induced transmembrane signaling events and cell activation. These results demonstrate that the Ti alpha and beta COOH termini containing the transmembrane domains are sufficient for structural and functional coupling of Ti to CD3.

摘要

T细胞抗原受体由识别抗原的Tiα/β异二聚体和被认为参与信号转导的相关CD3链组成。为了了解Ti与CD3之间相互作用的本质，将包含与CD8细胞外段相连的Tiα或β的COOH末端片段的嵌合分子转染到缺乏Tiβ链表达和细胞表面CD3的突变T细胞中。表达嵌合Ti和恢复CD3表面表达都需要两条嵌合链。CD8/Ti和CD3共同内化并共同免疫沉淀。嵌合受体的刺激诱导跨膜信号转导事件和细胞活化。这些结果表明，包含跨膜结构域的Tiα和β的COOH末端足以实现Ti与CD3的结构和功能偶联。

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1
Regions of the T cell receptor alpha and beta chains that are responsible for interactions with CD3.负责与CD3相互作用的T细胞受体α链和β链区域。
J Exp Med. 1991 May 1;173(5):1247-56. doi: 10.1084/jem.173.5.1247.
2
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引用本文的文献

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J Cell Biol. 1993 Jul;122(1):67-78. doi: 10.1083/jcb.122.1.67.
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本文引用的文献

1
Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.筛选表达编码黄嘌呤 - 鸟嘌呤磷酸核糖转移酶的大肠杆菌基因的动物细胞。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2072-6. doi: 10.1073/pnas.78.4.2072.
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OKT3: a monoclonal anti-human T lymphocyte antibody with potent mitogenic properties.OKT3：一种具有强大促有丝分裂特性的单克隆抗人T淋巴细胞抗体。
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3
Comparison of T3-associated 49- and 43-kilodalton cell surface molecules on individual human T-cell clones: evidence for peptide variability in T-cell receptor structures.个体人类T细胞克隆上与T3相关的49千道尔顿和43千道尔顿细胞表面分子的比较：T细胞受体结构中肽变异性的证据。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):4104-8. doi: 10.1073/pnas.80.13.4104.
4
A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains.一个人类T细胞特异性cDNA克隆编码一种与免疫球蛋白链具有广泛同源性的蛋白质。
Nature. 1984;308(5955):145-9. doi: 10.1038/308145a0.
5
The role of T3 surface molecules in the activation of human T cells: a two-stimulus requirement for IL 2 production reflects events occurring at a pre-translational level.T3表面分子在人T细胞激活中的作用：白细胞介素2产生的双刺激需求反映了翻译前水平发生的事件。
J Immunol. 1984 Jul;133(1):123-8.
6
Requirement for the coexpression of T3 and the T cell antigen receptor on a malignant human T cell line.人恶性T细胞系上T3与T细胞抗原受体共表达的要求。
J Exp Med. 1984 Nov 1;160(5):1284-99. doi: 10.1084/jem.160.5.1284.
7
Clonotypic structures involved in antigen-specific human T cell function. Relationship to the T3 molecular complex.参与抗原特异性人类T细胞功能的克隆型结构。与T3分子复合物的关系。
J Exp Med. 1983 Feb 1;157(2):705-19. doi: 10.1084/jem.157.2.705.
8
Does OKT3 monoclonal antibody react with an antigen-recognition structure on human T cells?OKT3单克隆抗体是否与人类T细胞上的抗原识别结构发生反应？
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1805-8. doi: 10.1073/pnas.78.3.1805.
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Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer.通过DNA转移重建活性表面T3/T细胞抗原受体。
Nature. 1985;316(6029):606-9. doi: 10.1038/316606a0.
10
Transmembrane signalling by the T cell antigen receptor. Perturbation of the T3-antigen receptor complex generates inositol phosphates and releases calcium ions from intracellular stores.T细胞抗原受体介导的跨膜信号传导。T3抗原受体复合物的扰动会产生肌醇磷酸，并从细胞内储存库释放钙离子。
J Exp Med. 1985 Mar 1;161(3):446-56. doi: 10.1084/jem.161.3.446.