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免疫球蛋白重链和CD3δ链基因增强子在造血祖细胞中对DNA酶I敏感。

Immunoglobulin heavy-chain and CD3 delta-chain gene enhancers are DNase I-hypersensitive in hemopoietic progenitor cells.

作者信息

Ford A M, Bennett C A, Healy L E, Navarro E, Spooncer E, Greaves M F

机构信息

Leukaemia Research Fund Centre, Chester Beatty Laboratories, London, United Kingdom.

出版信息

Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3424-8. doi: 10.1073/pnas.89.8.3424.

DOI:10.1073/pnas.89.8.3424
PMID:1533043
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC48880/
Abstract

Multipotential interleukin 3-dependent non-immortalized murine hemopoietic progenitor cells have DNase I-hypersensitive sites in the immunoglobulin heavy-chain and CD3 delta enhancers and transcribe germ-line T-cell antigen receptor gamma-chain (TCR gamma), but not IgM or TCR beta, genes. Induction of myeloid differentiation in these cells clones down expression and/or transcriptional accessibility of the immunoglobulin heavy-chain and TCR gamma genes. The CD3 delta enhancer region remains DNase I-hypersensitive but closes down in B cells. In embryonic stem cells and pan-mesodermal cells, these genes or enhancer regions are neither expressed nor DNase I-hypersensitive. These data suggest that lineage potential may be programmed, at least in part, by alterations in the accessibility or conformation of regulatory regions of genes and that some promiscuity of gene expression and/or accessibility can precede lineage commitment and maturation in progenitor cells induced to self-renew by interleukin 3.

摘要

多能白细胞介素3依赖的未永生化小鼠造血祖细胞在免疫球蛋白重链和CD3δ增强子中有脱氧核糖核酸酶I超敏位点，并转录种系T细胞抗原受体γ链（TCRγ）基因，但不转录IgM或TCRβ基因。这些细胞中髓系分化的诱导会克隆性下调免疫球蛋白重链和TCRγ基因的表达和/或转录可及性。CD3δ增强子区域仍然对脱氧核糖核酸酶I敏感，但在B细胞中关闭。在胚胎干细胞和全中胚层细胞中，这些基因或增强子区域既不表达也不对脱氧核糖核酸酶I敏感。这些数据表明，谱系潜能可能至少部分地由基因调控区域的可及性或构象改变所编程，并且在由白细胞介素3诱导自我更新的祖细胞中，在谱系定型和成熟之前可能存在一些基因表达和/或可及性的混杂情况。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02cf/48880/ef59cf043ed0/pnas01082-0273-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02cf/48880/d3aea9b53fbe/pnas01082-0271-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02cf/48880/2729a3f87b01/pnas01082-0272-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02cf/48880/ef59cf043ed0/pnas01082-0273-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02cf/48880/d3aea9b53fbe/pnas01082-0271-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02cf/48880/2729a3f87b01/pnas01082-0272-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/02cf/48880/ef59cf043ed0/pnas01082-0273-a.jpg

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本文引用的文献

1
Stromal cell associated haemopoiesis.基质细胞相关造血作用。
J Cell Physiol Suppl. 1982;1:87-94. doi: 10.1002/jcp.1041130414.
2
B220: a B cell-specific member of th T200 glycoprotein family.B220：T200糖蛋白家族的一种B细胞特异性成员。
Nature. 1981 Feb 19;289(5799):681-3. doi: 10.1038/289681a0.
3
The switch region associated with immunoglobulin C mu genes is DNase I hypersensitive in T lymphocytes.与免疫球蛋白Cμ基因相关的转换区在T淋巴细胞中对DNA酶I敏感。
基因表达的空间组织：活性染色质枢纽
Chromosome Res. 2003;11(5):447-59. doi: 10.1023/a:1024922626726.
4
Marrow stem cells shift gene expression and engraftment phenotype with cell cycle transit.骨髓干细胞随着细胞周期进程改变基因表达和植入表型。
J Exp Med. 2003 Jun 2;197(11):1563-72. doi: 10.1084/jem.20030031.
5
The new stem cell biology.新的干细胞生物学
Trans Am Clin Climatol Assoc. 2002;113:182-206; discussion 206-7.
6
Entry of amphotropic and 10A1 pseudotyped murine retroviruses is restricted in hematopoietic stem cell lines.嗜异性和10A1假型化鼠逆转录病毒进入造血干细胞系受到限制。
J Virol. 1998 Feb;72(2):1424-30. doi: 10.1128/JVI.72.2.1424-1430.1998.
7
Regulation of the myeloperoxidase enhancer binding proteins Pu1, C-EBP alpha, -beta, and -delta during granulocyte-lineage specification.粒细胞谱系特化过程中髓过氧化物酶增强子结合蛋白Pu1、C-EBPα、-β和-δ的调控
Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10838-43. doi: 10.1073/pnas.93.20.10838.
8
HEX: a novel homeobox gene expressed during haematopoiesis and conserved between mouse and human.HEX：一种在造血过程中表达且在小鼠和人类之间保守的新型同源框基因。
Nucleic Acids Res. 1993 Mar 11;21(5):1245-9. doi: 10.1093/nar/21.5.1245.
9
Dissecting a locus control region: facilitation of enhancer function by extended enhancer-flanking sequences.剖析一个基因座控制区：侧翼序列对增强子功能的促进作用
Mol Cell Biol. 1995 Feb;15(2):1123-35. doi: 10.1128/MCB.15.2.1123.
10
Activation of the beta-globin locus control region precedes commitment to the erythroid lineage.β-珠蛋白基因座控制区的激活先于向红系谱系的定向分化。
Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10618-22. doi: 10.1073/pnas.89.22.10618.
Nature. 1981 Nov 5;294(5836):90-2. doi: 10.1038/294090a0.
4
Heavy chain variable region contribution to the NPb family of antibodies: somatic mutation evident in a gamma 2a variable region.重链可变区对NPb抗体家族的贡献：γ2a可变区中明显的体细胞突变。
Cell. 1981 Jun;24(3):625-37. doi: 10.1016/0092-8674(81)90089-1.
5
"A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。附录
Anal Biochem. 1984 Feb;137(1):266-7. doi: 10.1016/0003-2697(84)90381-6.
6
Chromatin structure and protein binding in the putative regulatory region of the c-myc gene in Burkitt lymphoma.伯基特淋巴瘤中c-myc基因假定调控区域的染色质结构与蛋白质结合
Cell. 1984 Jun;37(2):381-91. doi: 10.1016/0092-8674(84)90368-4.
7
A conserved sequence in the immunoglobulin J kappa-C kappa intron: possible enhancer element.免疫球蛋白Jκ-Cκ内含子中的一个保守序列：可能的增强子元件。
Nature. 1983;304(5925):447-9. doi: 10.1038/304447a0.
8
Organization and reorganization of immunoglobulin genes in A-MULV-transformed cells: rearrangement of heavy but not light chain genes.A-MULV转化细胞中免疫球蛋白基因的组织与重排：重链基因而非轻链基因的重排。
Cell. 1981 Dec;27(2 Pt 1):381-90. doi: 10.1016/0092-8674(81)90421-9.
9
Continuous in vitro generation of multipotential stem cell clones from src-infected cultures.从src感染的培养物中持续体外生成多能干细胞克隆。
Nature. 1984;310(5974):228-30. doi: 10.1038/310228a0.
10
Ordered rearrangement of immunoglobulin heavy chain variable region segments.免疫球蛋白重链可变区片段的有序重排。
EMBO J. 1984 Jun;3(6):1209-19. doi: 10.1002/j.1460-2075.1984.tb01955.x.