Georgiou A S, Gil M A, Almiñana C, Cuello C, Vazquez J M, Roca J, Martinez E A, Fazeli A
Academic Unit of Reproductive and Developmental Medicine, The University of Sheffield, Jessop Wing, Tree Root Walk, SheffieldS, UK.
Reprod Domest Anim. 2011 Dec;46(6):1017-21. doi: 10.1111/j.1439-0531.2011.01777.x. Epub 2011 Mar 8.
Complement component 3 (C3) has well-established roles within immune system, but its roles outside of immune system are less characterized. The extensive presence of C3 throughout the female reproductive tract, and its temporal, and gamete-specific regulation of expression suggest a potential role for C3 in reproduction. In the present investigation, the effects of C3, C3b and iC3b on porcine oocyte maturation, fertilization and embryonic development were examined. We identified the ability of iC3b to positively influence oocyte maturation. No effects on fertilization efficiency, penetration rates, polyspermy and blastocyst formation were observed. However, C3, C3b and iC3b presence in embryo culture medium resulted in fewer total cells in test blastocysts compared to control blastocysts. The results of this study indicate a potential function for iC3b in oocyte maturation. Furthermore, it was demonstrated that the presence of either C3, C3b or iC3b has a negative influence on early embryonic development in the porcine species.
补体成分3（C3）在免疫系统中具有已明确的作用，但其在免疫系统之外的作用则较少被描述。C3广泛存在于女性生殖道中，并且其表达具有时间和配子特异性调控，这表明C3在生殖过程中可能发挥作用。在本研究中，研究了C3、C3b和iC3b对猪卵母细胞成熟、受精和胚胎发育的影响。我们发现iC3b具有促进卵母细胞成熟的能力。未观察到对受精效率、穿透率、多精受精和囊胚形成的影响。然而，与对照囊胚相比，胚胎培养基中存在C3、C3b和iC3b会导致测试囊胚中的总细胞数减少。本研究结果表明iC3b在卵母细胞成熟中具有潜在功能。此外，还证明了C3、C3b或iC3b的存在对猪早期胚胎发育具有负面影响。
