条件性敲除小鼠 Gata4 和 Fog2 基因揭示了它们在哺乳动物性别分化中的不同作用。
Conditional ablation of Gata4 and Fog2 genes in mice reveals their distinct roles in mammalian sexual differentiation.
机构信息
Department of Genetics, Dartmouth Medical School, Hanover, NH 03755, USA.
出版信息
Dev Biol. 2011 May 15;353(2):229-41. doi: 10.1016/j.ydbio.2011.02.032. Epub 2011 Mar 6.
Abstract
Assembly of functioning testis and ovary requires a GATA4-FOG2 transcriptional complex. To define the separate roles for GATA4 and FOG2 proteins in sexual development of the testis we have ablated the corresponding genes in somatic gonadal cells. We have established that GATA4 is required for testis differentiation, for the expression of Dmrt1 gene, and for testis cord morphogenesis. While Sf1Cre-mediated excision of Gata4 permitted normal expression of most genes associated with embryonic testis development, gonadal loss of Fog2 resulted in an early partial block in male pathway and sex reversal. We have also determined that testis sexual differentiation is sensitive to the timing of GATA4 loss during embryogenesis. Our results now demonstrate that these two genes also have non-overlapping essential functions in testis development.
摘要
形成有功能的睾丸和卵巢需要 GATA4-FOG2 转录复合物。为了明确 GATA4 和 FOG2 蛋白在睾丸性发育中的独立作用，我们已经在体生殖细胞中敲除了相应的基因。我们已经证实 GATA4 对于睾丸分化、Dmrt1 基因的表达以及睾丸索形态发生是必需的。虽然 Sf1Cre 介导的 Gata4 缺失允许与胚胎睾丸发育相关的大多数基因正常表达，但 Fog2 的生殖细胞缺失导致雄性途径的早期部分阻断和性别反转。我们还确定了睾丸性分化对胚胎发生过程中 GATA4 缺失时机的敏感性。我们的结果现在表明，这两个基因在睾丸发育中也具有非重叠的必需功能。
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