College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, PR China.
Virus Res. 2011 Jun;158(1-2):33-6. doi: 10.1016/j.virusres.2011.03.001. Epub 2011 Mar 6.
In the present study, seven new defective interfering (DI) RNA species of porcine reproductive and respiratory syndrome virus (PRRSV) were identified. RT-PCR, Northern blot and sequence analyses indicated that these DI RNA specie have deletions of 8513-9176 nucleotides located between Nsp1/Nsp2 and Nsp10. Compared with the previous DI RNAs of PRRSV reported, they have three distinct characteristics: much smaller deletion sizes; different nucleotide repeats (2-12nt) used in the junction sites and in-frame deletions. The results further suggested that the similarity-assisted RNA recombination may be the main cause of generation of DI RNAs in PRRSV and probably in other arteriviruses.
在本研究中，鉴定了七种新的猪繁殖与呼吸综合征病毒（PRRSV）缺陷干扰（DI）RNA 。RT-PCR、Northern blot 和序列分析表明，这些 DI RNA 具有 Nsp1/Nsp2 和 Nsp10 之间 8513-9176 个核苷酸的缺失。与之前报道的 PRRSV DI RNA 相比，它们具有三个明显的特征：更小的缺失大小、不同的核苷酸重复（2-12nt）用于连接位点和框架内缺失。结果进一步表明，相似性辅助的 RNA 重组可能是 PRRSV 以及可能是其他动脉炎病毒中产生 DI RNA 的主要原因。
