秀丽隐杆线虫增强 RNA 干扰突变体的组织特异性。
Tissue specificity of Caenorhabditis elegans enhanced RNA interference mutants.
机构信息
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
出版信息
Genetics. 2011 May;188(1):235-7. doi: 10.1534/genetics.111.127209. Epub 2011 Mar 8.
Abstract
Gene knockdown by RNA interference (RNAi) in Caenorhabditis elegans is readily achieved by feeding bacteria expressing double-stranded RNA (dsRNA). Enhanced RNAi (Eri) mutants facilitate RNAi due to their hypersensitivity to dsRNA. Here, we compare eight Eri mutants for sensitivity to ingested dsRNA, targeting a variety of tissue-specific genes.
摘要
利用 RNA 干扰（RNAi）使基因失活在秀丽隐杆线虫中很容易通过喂食表达双链 RNA（dsRNA）的细菌来实现。增强型 RNAi（Eri）突变体由于对 dsRNA 的超敏感性而促进 RNAi。在这里，我们比较了八种 Eri 突变体对各种组织特异性基因的 dsRNA 的摄取敏感性。
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