Hammell Christopher M, Hannon Gregory J
Cold Spring Harb Protoc. 2016 Jan 4;2016(1):pdb.prot086306. doi: 10.1101/pdb.prot086306.
In Caenorhabditis elegans, long double-stranded RNAs (dsRNAs) are overwhelmingly the trigger of choice for inducing RNA interference (RNAi). Although injection of dsRNA into the somatic or germline tissues of animals requires both specific equipment and technical skills, the ability of C. elegans to amplify the initial dsRNA trigger and to transmit the RNAi activity to other somatic tissues and to the progeny of injected animals is one of the main advantages of using C. elegans as a model system. The direct injection of dsRNA into parental animals is the most reliable method for RNAi and also presents the least experiment-to-experiment and animal-to-animal variability.
在秀丽隐杆线虫中，长双链RNA（dsRNA）是诱导RNA干扰（RNAi）的首选触发物。虽然将dsRNA注射到动物的体细胞或生殖组织中既需要特定设备又需要技术技能，但秀丽隐杆线虫能够扩增初始dsRNA触发物并将RNAi活性传递到其他体细胞组织以及注射动物的后代，这是将秀丽隐杆线虫用作模型系统的主要优势之一。将dsRNA直接注射到亲代动物中是进行RNAi最可靠的方法，并且在不同实验之间以及不同动物之间的变异性也是最小的。
