Department of Physiology and Metabolism, University of Geneva, Geneva, Switzerland.
J Phys Condens Matter. 2010 May 19;22(19):194101. doi: 10.1088/0953-8984/22/19/194101. Epub 2010 Apr 26.
The measurement of cell adhesion using single cell force spectroscopy methods was compared with earlier methods for measuring cell adhesion. This comparison provided a means and rationale for separating components of the measurement retract curve that were due to interactions between the substrate and the glycocalyx, and interactions that were due to cell surface integrins binding to a substrate-bound ligand. The glycocalyx adhesion was characterized by multiple jumps with dispersed jump sizes that extended from 5 to 30 µm from the origin. The integrin mediated adhesion was represented by the F(max) (maximum detachment force), was generally within the first 5 µm and commonly detached with a single rupture cascade. The integrin peak (F(max)) increases with time and the rate of increase shows large cell to cell variability with a peak ∼ 50 nN s(-1) and an average rate of increase of 75 pN s(-1). This is a measure of the rate of increase in the number of adhesive integrin-ligand bonds/cell as a function of contact time.
使用单细胞力谱法测量细胞黏附力与早期测量细胞黏附力的方法进行了比较。这种比较为分离由于基质和糖萼之间的相互作用以及由于细胞表面整合素与基质结合配体结合而导致的回缩曲线的测量提供了一种手段和原理。糖萼黏附力的特征是具有分散的跳跃大小的多次跳跃，从原点延伸 5 到 30 µm。整合素介导的黏附由 F(max)（最大分离力）表示，通常在最初的 5 µm 内，并且通常通过单个断裂级联脱离。整合素峰（F(max)）随时间增加，增加速率显示出细胞间的大的可变性，峰值约为 50 nN s(-1)，平均增加速率为 75 pN s(-1)。这是测量随着接触时间的增加，黏附整合素-配体键/细胞的数量增加的速率的一种方法。
