Semmekrot B A, Wiesel P H, Monnens L A, Guignard J P
Unité de Néphrologie, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
Life Sci. 1990;46(12):849-56. doi: 10.1016/0024-3205(90)90114-7.
Plasma levels of atrial natriuretic peptide (ANP) and the effect of exogenous ANP on renal function have been studied in newborn and adult rabbits. In order to investigate an age difference in responsiveness to ANP, we studied the renal effects of alpha-human ANP (1-28) administered at the same dose per kg body weight in adult and neonatal rabbits. Plasma basal ANP levels were similar in 18 newborn (4- to 11-day-old) compared to 7 adult rabbits (150 +/- 16 and 151 +/- 28 pg/ml, resp.). Eleven newborn and 11 adult rabbits were anesthetized and mechanically ventilated. After a control period, each animal received an hANP loading dose (3 micrograms/kg i.v.), followed by an infusion of 0.3 micrograms/kg/min. Blood gases remained stable throughout the experiment in both groups. Mean blood pressure decreased in newborn (28.5 +/- 0.8 to 26.2 +/- 1.0 mmHg) and adult (92 +/- 3 to 84 +/- 3 mmHg) animals. Percent hANP-induced changes in renal functions in newborn and adult rabbits were, respectively: urine flow rate: -21 +/- 4% and +57 +/- 8%; urinary sodium excretion: +4 +/- 7% and +81 +/- 11%; glomerular filtration rate (GFR): -19 +/- 4% and -4 +/- 6%; renal blood flow (RBF): -22 +/- 4% and -11 +/- 5%. As expected, diuresis and natriuresis increased in adult rabbits. Failure of hANP to increase natriuresis and diuresis in newborn rabbits could be related to the marked decrease in GFR, receptor immaturity and/or interactions with other hormonal systems.
已在新生兔和成年兔中研究了血浆心房利钠肽（ANP）水平以及外源性ANP对肾功能的影响。为了研究对ANP反应性的年龄差异，我们在成年兔和新生兔中以每千克体重相同剂量给予α-人ANP（1-28），研究其对肾脏的影响。18只新生兔（4至11日龄）与7只成年兔相比，血浆基础ANP水平相似（分别为150±16和151±28 pg/ml）。11只新生兔和11只成年兔接受麻醉并进行机械通气。在对照期后，每只动物静脉注射hANP负荷剂量（3微克/千克），随后以0.3微克/千克/分钟的速度输注。两组在整个实验过程中血气均保持稳定。新生兔（从28.5±0.8降至26.2±1.0 mmHg）和成年兔（从92±3降至84±3 mmHg）的平均血压均下降。hANP诱导的新生兔和成年兔肾功能变化百分比分别为：尿流率：-21±4%和+57±8%；尿钠排泄：+4±7%和+81±11%；肾小球滤过率（GFR）：-19±4%和-4±6%；肾血流量（RBF）：-22±4%和-11±5%。正如预期的那样，成年兔的利尿和利钠作用增强。hANP未能增加新生兔的利钠和利尿作用可能与GFR显著降低、受体不成熟和/或与其他激素系统的相互作用有关。
