Scalinci Sergio Zaccaria, Scorolli Lucia, Meduri Alessandro, Grenga Pier Luigi, Corradetti Giulia, Metrangolo Cristian
Low Vision Center - University of Bologna, Bologna, Italy;
Clin Ophthalmol. 2011;5:215-21. doi: 10.2147/OPTH.S16866. Epub 2011 Feb 16.
To evaluate the role of prepared basic fibroblast growth factor (bFGF) and cytochrome c peroxidase (CCP) combination eyedrops in corneal epithelial healing of transgenic mice (B6(A)-Rpe(rd12)/J) after excimer laser photoablation.
In this prospective study, 216 eyes of 108 mice underwent bilateral photorefractive keratectomy. We considered 4 groups: A, B, C, and D. Group A received standard topical postoperative therapy with tobramycin, diclofenac, and dexamethasone eyedrops plus CCP at 3 drops per day for a week or until corneal re-epithelialization was achieved. Group B received standard topical postoperative therapy plus bFGF eyedrops and phosphate-buffered saline (PBS) 3 drops per day for a week or until corneal re-epithelialization was complete. In group C, 1 eye received standard topical postoperative therapy plus CCP eyedrops, bFGF eyedrops, and PBS 3 drops per day for a week or until corneal re-epithelialization was complete. Control eyes (group D) received a standard topical postoperative therapy plus placebo eyedrops. Mice were followed-up for a week from the day after the surgery to evaluate the rate of corneal re-epithelialization.
Data were analyzed by ANOVA using the XLSTAT 2010 software. Eyes in group A, B, and C healed completely before the fifth postoperative day, achieving, respectively, a re-epithelialization time of 92 hours ± 10 SD, 90 hours ± 12 SD, and 86 hours ± 12 SD. Group D had a re-epithelialization time of 121 hours ± 8 SD (P < 0.05). No side effects or toxic effects were documented.
Results suggest that re-epithelialization after phototherapeutic keratectomy can benefit from topical therapy with CCP/bFGF combination eyedrops. Further clinical studies are needed to evaluate the long-term effectiveness of these eyedrops to prevent corneal haze.
评估制备好的碱性成纤维细胞生长因子（bFGF）和细胞色素c过氧化物酶（CCP）联合滴眼液对准分子激光光消融术后转基因小鼠（B6(A)-Rpe(rd12)/J）角膜上皮愈合的作用。
在这项前瞻性研究中，108只小鼠的216只眼睛接受了双侧光性屈光性角膜切削术。我们将其分为4组：A组、B组、C组和D组。A组术后接受标准局部治疗，使用妥布霉素、双氯芬酸和地塞米松滴眼液，外加CCP，每天3滴，持续一周或直至角膜重新上皮化。B组接受标准局部术后治疗加bFGF滴眼液和磷酸盐缓冲盐水（PBS），每天3滴，持续一周或直至角膜重新上皮化完成。C组中，1只眼睛接受标准局部术后治疗加CCP滴眼液、bFGF滴眼液和PBS，每天3滴，持续一周或直至角膜重新上皮化完成。对照眼（D组）接受标准局部术后治疗加安慰剂滴眼液。从手术后第二天起对小鼠进行为期一周的随访，以评估角膜重新上皮化的速率。
使用XLSTAT 2010软件通过方差分析对数据进行分析。A组、B组和C组的眼睛在术后第五天前完全愈合，重新上皮化时间分别为92小时±10标准差、90小时±12标准差和86小时±12标准差。D组的重新上皮化时间为121小时±8标准差（P<0.05）。未记录到副作用或毒性作用。
结果表明，光性角膜切削术后的重新上皮化可受益于CCP/bFGF联合滴眼液的局部治疗。需要进一步的临床研究来评估这些滴眼液预防角膜混浊的长期有效性。
