Laboratory of Experimental Virology, Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
PLoS One. 2011 Feb 28;6(2):e17316. doi: 10.1371/journal.pone.0017316.
Dendritic cells bind an array of antigens and DC-SIGN has been postulated to act as a receptor for mucosal pathogen transmission. Bile salt-stimulated lipase (BSSL) from human milk potently binds DC-SIGN and blocks DC-SIGN mediated trans-infection of CD4(+) T-lymphocytes with HIV-1. Objective was to study variation in DC-SIGN binding properties and the relation between DC-SIGN binding capacity of milk and BSSL gene polymorphisms.
ELISA and PCR were used to study DC-SIGN binding properties and BSSL exon 11 size variation for human milk derived from 269 different mothers distributed over 4 geographical regions.
DC-SIGN binding properties were highly variable for milks derived from different mothers and between samplings from different geographical regions. Differences in DC-SIGN binding were correlated with a genetic polymorphism in BSSL which is related to the number of 11 amino acid repeats at the C-terminus of the protein.
The observed variation in DC-SIGN binding properties among milk samples may have implications for the risk of mucosal transmission of pathogens during breastfeeding.
树突状细胞结合了一系列抗原，而 DC-SIGN 被认为是黏膜病原体传播的受体。人乳中的胆盐刺激脂肪酶 (BSSL) 能够强烈结合 DC-SIGN，并阻断 DC-SIGN 介导的 HIV-1 对 CD4+T 淋巴细胞的转染。目的是研究 DC-SIGN 结合特性的变化以及母乳中 DC-SIGN 结合能力与 BSSL 基因多态性之间的关系。
使用 ELISA 和 PCR 研究了 269 位来自 4 个地理区域的不同母亲的母乳中 DC-SIGN 结合特性和 BSSL 外显子 11 大小的变化。
来自不同母亲的母乳以及不同地理区域的采样之间的 DC-SIGN 结合特性差异很大。DC-SIGN 结合的差异与 BSSL 中的遗传多态性相关，该多态性与蛋白 C 末端的 11 个氨基酸重复数有关。
母乳样本中观察到的 DC-SIGN 结合特性的变化可能会影响母乳喂养期间病原体经黏膜传播的风险。
