蛋白质-DNA 相互作用的核磁共振分析。
Nuclear magnetic resonance analysis of protein-DNA interactions.
机构信息
Université de Toulouse, UPS, Department of Structural Biology and Biophysics, F-31077 Toulouse, France.
出版信息
J R Soc Interface. 2011 Aug 7;8(61):1065-78. doi: 10.1098/rsif.2010.0543. Epub 2011 Mar 9.
Abstract
Recent methodological and instrumental advances in solution-state nuclear magnetic resonance have opened up the way to investigating challenging problems in structural biology such as large macromolecular complexes. This review focuses on the experimental strategies currently employed to solve structures of protein-DNA complexes and to analyse their dynamics. It highlights how these approaches can help in understanding detailed molecular mechanisms of target recognition.
摘要
近年来，溶液核磁共振技术在方法学和仪器方面的进展为解决结构生物学中的难题提供了可能，例如研究大型的生物大分子复合物。本综述重点介绍了目前用于解析蛋白-DNA 复合物结构及其动力学的实验策略，并强调了这些方法如何帮助我们理解靶标识别的详细分子机制。
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