Mattsson E, Brunkwall J, Bergqvist D
Department of Surgery, University of Lund, Malmö General Hospital, Sweden.
Eur J Vasc Surg. 1990 Feb;4(1):11-7. doi: 10.1016/s0950-821x(05)80033-7.
Vasospasm and thrombosis complicate percutaneous transluminal angioplasty (PTA). To study if the release of the prostanoids PGI2 and TxA2 are affected by PTA, the following experiment was undertaken: In ten rabbits, the upper or lower half of the aorta was randomised either to transluminal angioplasty or control segment. After excision the segments were simultaneously but separately perfused ex vivo with Hank's balanced salt solution for five consecutive 15 min periods. Arachidonic acid was added to the perfusate for the last 15 min period. PGI2 and TxA2 were measured by radioimmunoassay in the perfusate as the stable degradation products 6-keto-PGF1 alpha and TxB2. After perfusion, the two aortic segments were prepared for scanning electron microscopy (SEM). Angioplasty decreased the basic release of PGI2 as well as the response to arachidonic acid. This is likely to be due to endothelial denudation as seen by SEM. The release of TxA2 from the vessel wall was very low and was not increased by dilatation. The influence of angioplasty on the prostanoid system may be of importance in the complications of vasospasm and thrombosis.
血管痉挛和血栓形成使经皮腔内血管成形术（PTA）变得复杂。为了研究前列腺素PGI2和TxA2的释放是否受PTA影响，进行了以下实验：在十只兔子中，将主动脉的上半部分或下半部分随机分为经腔血管成形术组或对照组。切除后，将这些节段同时但分别在体外用汉克平衡盐溶液连续灌注五个15分钟时间段。在最后15分钟时间段向灌注液中加入花生四烯酸。通过放射免疫分析法测定灌注液中作为稳定降解产物6-酮-PGF1α和TxB2的PGI2和TxA2。灌注后，将两个主动脉节段制备用于扫描电子显微镜（SEM）检查。血管成形术降低了PGI2的基础释放以及对花生四烯酸的反应。这可能是由于SEM所见的内皮剥脱。血管壁中TxA2的释放非常低，并且不会因扩张而增加。血管成形术对前列腺素系统的影响可能在血管痉挛和血栓形成的并发症中具有重要意义。
