Department of Pharmacology, College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
Otol Neurotol. 2011 Jul;32(5):872-6. doi: 10.1097/MAO.0b013e31821341ac.
The purpose of this study was to evaluate the relations among the combined therapy with steroid and the detoxification enzyme gene polymorphisms in patients with sudden sensorineural hearing loss (SSNHL). The pathogenetic mechanism of inner ear dysfunction could involve an increase in lipid peroxidation and a decrease in cellular antioxidant defense. Glutathione S-transferases (GSTs) and cytochrome P450 (CYP) belong to a system of detoxification and antioxidant enzymes that have been demonstrated in the inner ear.
A prospective study in patients with SSNHL.
All 441 subjects were genotyped for GSTM1, GSTT1, and CYP1A1 polymorphisms. The polymorphisms were analyzed by polymerase chain reaction amplification, restriction enzyme digestion, and deoxyribonucleic acid fragment separation by electrophoresis.
No significant difference was observed between SSNHL patients and controls in 3 polymorphisms. However, the prevalence of the partial recovery group in patients with the CC genotype of CYP1A1 (22%) was higher than that in the complete recovery (7.4%) or no recovery group (12.5%) for the subjects classified according to modified Siegel's criteria but were not statistically significant.
This is the first approach to analyze gene polymorphism and efficacy of clinical treatment of patients with SSNHL, although the observations do not confirm the effect of the GSTM1/T1 and CYP1A1 genotypes as a risk factor for SSNHL.
本研究旨在评估类固醇联合治疗与突发性聋（SSNHL）患者解毒酶基因多态性之间的关系。内耳功能障碍的发病机制可能涉及脂质过氧化增加和细胞抗氧化防御减少。谷胱甘肽 S-转移酶（GSTs）和细胞色素 P450（CYP）属于解毒和抗氧化酶系统，已在内耳中得到证实。
对 SSNHL 患者进行前瞻性研究。
对 441 名受试者进行 GSTM1、GSTT1 和 CYP1A1 多态性的基因分型。通过聚合酶链反应扩增、限制性内切酶消化和电泳分离脱氧核糖核酸片段分析多态性。
在 3 种多态性中，SSNHL 患者与对照组之间无显著差异。然而，根据改良的 Siegel 标准对患者进行分类后，CYP1A1 基因型为 CC 的部分恢复组（22%）的患病率高于完全恢复组（7.4%）或无恢复组（12.5%），但无统计学意义。
这是首次分析 SSNHL 患者基因多态性和临床治疗效果的方法，尽管观察结果不能证实 GSTM1/T1 和 CYP1A1 基因型作为 SSNHL 的危险因素的作用。
