College of Korean Medicine, Institute of Korean Medicine, Kyung Hee University, 1 Heogi-dong, Dongdaemun-gu, Seoul, Republic of Korea.
Immunopharmacol Immunotoxicol. 2013 Feb;35(1):52-6. doi: 10.3109/08923973.2012.719523. Epub 2012 Sep 26.
The cause and pathogenesis of sudden sensorineural hearing loss (SSNHL) remain unknown. IL-1β is one of the most powerful inflammatory cytokines. The aim of this study was to evaluate the relationships between interleukin-1 β (IL-1β) gene polymorphisms (-511 C/T and +3953 C/T) in patients with SSNHL. One hundred two patients affected by SSNHL and 595 controls were genotyped for IL-1β gene polymorphisms. The polymorphisms were analyzed by polymerase chain reaction amplification and DNA fragment separation via electrophoresis. Compared to controls, the IL-1β (+3953) T allele increased the relative risk of SSNHL in subjects with IL-1β (-511) TT genotype (p = 0.022, OR = 9.111, 95% CI = 1.441-57.618). In this study, polymorphisms in the IL-1β -511 and IL-1β +3953 loci were assessed for evidence of association with SSNHL. From this assessment, a significant difference in carriage of both the IL-1β -511 T allele and the IL-1β +3953 T allele was observed between SSNHL and controls. This suggests that the IL-1β -511 and +3953 loci may play an important role in the etiopathogenesis of SSNHL.
突发性聋（SSNHL）的病因和发病机制尚不清楚。IL-1β 是最强的炎症细胞因子之一。本研究旨在评估白细胞介素-1β（IL-1β）基因多态性（-511C/T 和 +3953C/T）与 SSNHL 患者的关系。102 例 SSNHL 患者和 595 例对照者进行了 IL-1β 基因多态性的基因分型。采用聚合酶链反应扩增和电泳分离 DNA 片段分析多态性。与对照组相比，IL-1β（+3953）T 等位基因增加了 IL-1β（-511）TT 基因型患者 SSNHL 的相对风险（p=0.022，OR=9.111，95%CI=1.441-57.618）。在这项研究中，评估了 IL-1β-511 和 IL-1β+3953 位点的多态性与 SSNHL 相关的证据。从这项评估中，SSNHL 患者和对照组携带 IL-1β-511T 等位基因和 IL-1β+3953T 等位基因的比例存在显著差异。这表明 IL-1β-511 和+3953 位点可能在 SSNHL 的发病机制中发挥重要作用。
