Duncan D R, Widholm J M
University of Illinois, Urbana, IL.
Methods Mol Biol. 1990;6:29-37. doi: 10.1385/0-89603-161-6:29.
Experiments using plant tissue cultures, such as measuring their tolerance to herbicides or metabolite analogs, elucidating biochemical pathways by exposing the tissues to metabolic inhibitors, or cryopreserving the cultures, require at some point the determination of the tissue's viability. Viability is the capacity of a cell or organism to live, and the state of being viable is most commonly recognized by the growth of the studied tissues (1-3). Measurements of growth can include measuring increases in fresh or dry weight of a cultured tissue (2,3) or measuring increases in cell number or size (2-4).
使用植物组织培养进行的实验,例如测量它们对除草剂或代谢物类似物的耐受性、通过将组织暴露于代谢抑制剂来阐明生化途径,或冷冻保存培养物,在某些时候需要确定组织的活力。活力是细胞或生物体存活的能力,而存活状态最通常通过所研究组织的生长来识别(1 - 3)。生长的测量可以包括测量培养组织鲜重或干重的增加(2,3)或测量细胞数量或大小的增加(2 - 4)。