Thomas S, Coffin R S, Watts P, Gough G, Latchman D S
Department of Molecular Pathology, Windeyer Institute of Medical Sciences, University College London Medical School, United Kingdom.
J Virol. 1998 Apr;72(4):3495-500. doi: 10.1128/JVI.72.4.3495-3500.1998.
The TAATGARAT motif in the herpes simplex virus (HSV) immediate-early (IE) gene promoters plays a key role in their activation by the Oct-1-Vmw65 complex, but its role in mediating inhibitory effects of cellular octamer-binding proteins is less clear. We have used indicator viruses containing reporter constructs with different IE promoters driving a reporter beta-galactosidase gene within the viral genome to investigate this. We showed that deletion of the upstream IE promoter region containing the TAATGARAT motifs abolishes the inhibitory effect of the cellular octamer-binding proteins Oct-2.4 and Oct-2.5 on the viral IE promoter. This inhibitory effect can be restored by addition of a single TAATGARAT motif to the minimal promoter within the viral genome. Hence, the TAATGARAT motif can indeed mediate both positive and negative effects of cellular transcription factors when it is located within the viral genome.
单纯疱疹病毒（HSV）立即早期（IE）基因启动子中的TAATGARAT基序在被Oct-1-Vmw65复合物激活过程中起关键作用，但其在介导细胞八聚体结合蛋白抑制作用方面的作用尚不清楚。我们使用了指示病毒，其含有在病毒基因组内驱动报告β-半乳糖苷酶基因的不同IE启动子的报告构建体来对此进行研究。我们发现，缺失包含TAATGARAT基序的上游IE启动子区域可消除细胞八聚体结合蛋白Oct-2.4和Oct-2.5对病毒IE启动子的抑制作用。通过向病毒基因组内的最小启动子添加单个TAATGARAT基序可恢复这种抑制作用。因此，当TAATGARAT基序位于病毒基因组内时，它确实可以介导细胞转录因子的正负两种效应。
