Emery V C
Department of Virology, Royal Free Hospital School of Medicine, Hampstead, London, UK.
Methods Mol Biol. 1992;8:309-18. doi: 10.1385/0-89603-191-8:309.
This chapter aims to provide the reader with the experimental protocols required to produce a baculovirus containing the gene of interest, including transfection of Spodoptera frugiperda cells and the screening of progeny virus by plaque assay. In addition, an outline of the biochemical methods routinely used to characterize the expressed protein product within the baculovirus system will be given. Throughout the experimental procedures, areas that researchers generally find technically difficult will be highlighted in the hope that this may ameliorate some of the problems encountered in using the system ab initio.
本章旨在为读者提供制备含目的基因杆状病毒所需的实验方案，包括转染草地贪夜蛾细胞以及通过噬斑测定法筛选子代病毒。此外，还将概述在杆状病毒系统中常规用于表征表达的蛋白质产物的生化方法。在整个实验过程中，将突出研究人员通常认为技术难度较大的方面，希望这能缓解一些从头开始使用该系统时遇到的问题。
