Department of Chemistry, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia.
Nat Prod Res. 2011 Mar;25(5):496-510. doi: 10.1080/14786411003792165.
A comprehensive investigation of the chemical composition of the aerial parts of Hypericum perforatum L. collected in three habitations in Estonia was carried out. An analysis by gas chromatography-mass spectrometry and gas chromatography-flame ionisation detection established the main components of the essential oils. The phenolic compounds both in ethanol and water extracts of the plant were analysed using liquid chromatography-mass spectrometry (LC-MS) and capillary zone electrophoresis. In addition to the earlier published polyphenols, several new phenolic acids and flavonoids, such as quercetin hexoside malonates and an A-type catechin-epicatechin trimer were identified in this Hypericum for the first time. The contents of the pharmaceutically important antidepressants hyperforin and hypericin were also estimated by LC-MS and compared with the data in the literature. The composition of the mineral elements was determined by atomic absorption spectroscopy. The results of the study demonstrate a rather high variability in the content of different substance groups in H. perforatum L. and, hence, the need for a survey of the raw material in the course of selection of raw materials for pharmaceutical preparations.
对在爱沙尼亚三个栖息地采集的贯叶连翘地上部分的化学成分进行了全面研究。气相色谱-质谱和气相色谱-火焰离子化检测分析确定了精油的主要成分。采用液相色谱-质谱（LC-MS）和毛细管区带电泳法分析了植物乙醇和水提取物中的酚类化合物。除了之前发表的多酚外，还首次在该贯叶连翘中鉴定出几种新的酚酸和类黄酮，如槲皮素六糖苷丙二酸盐和 A 型儿茶素-表儿茶素三聚体。还通过 LC-MS 估算了具有重要药用价值的抗抑郁药金丝桃素和圣约翰草素的含量，并与文献中的数据进行了比较。通过原子吸收光谱法测定了矿物质元素的组成。研究结果表明，贯叶连翘中不同物质组的含量存在相当大的变异性，因此在选择制药原料时需要对原料进行调查。
