Males Zeljan, Brantner Adelheid H, Sović Katarina, Pilepić Kroata Hazler, Plazibat Misko
Department of Pharmaceutical Botany, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia.
Acta Pharm. 2006 Sep;56(3):359-67.
The aerial parts of H. perforatum subsp. perforatum and H. perforatum subsp. angustifolium were investigated for their chemical composition and antimicrobial activity. Spectrophotometric analysis indicated that H. perforatum subsp. perforatum is richer in flavonoids and tannins than the other subspecies. HPLC analysis confirmed the higher yield of flavonoids in H. perforatum subsp. perforatum and gave also a higher content of phenolic acids. H. perforatum subsp. angustifolium contained more hypericin. The presence of rutin was proven only in H. perforatum subsp. perforatum. The antimicrobial activity of the extracts of both subspecies was evaluated based on the inhibition zone diameters using the hole-plate diffusion method. The MeOH extracts, dichloromethane and petroleum ether fractions were effective against Staphylococcus aureus, S. epidermidis, Enterococcus faecalis and Bacillus subtilis. The results indicate that H. perforatum subsp. angustifolium had a stronger antimicrobial effect than the other subspecies.
对贯叶连翘亚种贯叶连翘和狭叶贯叶连翘的地上部分进行了化学成分和抗菌活性研究。分光光度分析表明，贯叶连翘亚种贯叶连翘比其他亚种富含黄酮类化合物和单宁。高效液相色谱分析证实，贯叶连翘亚种贯叶连翘中黄酮类化合物的产量更高，酚酸含量也更高。狭叶贯叶连翘含有更多的金丝桃素。仅在贯叶连翘亚种贯叶连翘中证实存在芦丁。使用孔板扩散法，基于抑菌圈直径评估了两个亚种提取物的抗菌活性。甲醇提取物、二氯甲烷和石油醚馏分对金黄色葡萄球菌、表皮葡萄球菌、粪肠球菌和枯草芽孢杆菌有效。结果表明，狭叶贯叶连翘的抗菌效果比其他亚种更强。
