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中欧苔草属(莎草科)选定物种酚酸的反相高效液相色谱分析及其分类学意义

RP-HPLC analysis of phenolic acids of selected Central European Carex L. (Cyperaceae) species and its implication for taxonomy.

作者信息

Bogucka-Kocka Anna, Szewczyk Katarzyna, Janyszek Magdalena, Janyszek Sławomir, Cieśla Łukasz

机构信息

Medical University of Lublin, Department of Pharmaceutical Botany, Chodźki 1 St, 20-093 Lublin, Poland.

出版信息

J AOAC Int. 2011 Jan-Feb;94(1):9-16.

PMID:21391476
Abstract

Eighteen species belonging to the Carex genus were checked for the presence and the amount of eight phenolic acids (p-hydroxybenzoic, vanillic, caffeic, syringic, protocatechuic, p-coumaric, sinapic, and ferulic) by means of HPLC. Both the free and bonded phenolic acids were analyzed. The majority of the analyzed acids occurred in the studied species in relatively high amounts. The highest concentrations found were caffeic acid and p-coumaric acid, for which the detected levels were negatively correlated. A very interesting feature was the occurrence of sinapic acid, a compound very rarely detected in plant tissues. Its distribution across the analyzed set of species can be hypothetically connected with the humidity of plants' habitats. Several attempted tests of aggregative cluster analysis showed no similarity to the real taxonomical structure of the genus Carex. Thus, the phenolic acids' composition cannot be considered as the major taxonomical feature for the genus Carex.

摘要

通过高效液相色谱法(HPLC)对莎草属的18个物种进行了检测,以确定8种酚酸(对羟基苯甲酸、香草酸、咖啡酸、丁香酸、原儿茶酸、对香豆酸、芥子酸和阿魏酸)的存在情况及含量。同时分析了游离酚酸和结合酚酸。在所研究的物种中,大多数被分析的酸含量相对较高。检测到的最高浓度的酸是咖啡酸和对香豆酸,其检测水平呈负相关。一个非常有趣的特征是芥子酸的出现,这种化合物在植物组织中很少被检测到。它在分析的物种组中的分布可能与植物栖息地的湿度有关。几次聚合聚类分析尝试均表明与莎草属的实际分类结构没有相似性。因此,酚酸组成不能被视为莎草属的主要分类特征。

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