School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.
J Agric Food Chem. 2011 Apr 13;59(7):3295-305. doi: 10.1021/jf1040959. Epub 2011 Mar 10.
Stable isotope ratio analysis (SIRA) was used as an analytical tool to verify the preslaughter diet of beef cattle. Muscle and tail hair samples were collected from animals fed either pasture (P), a barley-based concentrate (C), silage followed by pasture (SiP), or silage followed by pasture with concentrate (SiPC) for 1 year (n = 25 animals per treatment). The (13)C/(12)C, (15)N/(14)N, (2)H/(1)H, and (34)S/(32)S isotope ratios in muscle clearly reflected those of the diets consumed by the animals. By applying a stepwise canonical discriminant analysis, a good discrimination of bovine meat according to dietary regimen was obtained. On the basis of the classification success rate, the (13)C/(12)C and (34)S/(32)S ratios in muscle were the best indicators for authentication of beef from animals consuming the different diets. Analysis of (13)C/(12)C and (15)N/(14)N in tail hair sections provided an archival record of changes to the diet of the cattle for periods of over 1 year preslaughter.
稳定同位素比分析（SIRA）被用作一种分析工具，以验证肉牛的宰前饮食。从放牧（P）、以大麦为基础的浓缩饲料（C）、青贮料后放牧（SiP）或青贮料后放牧加浓缩饲料（SiPC）饲养 1 年的动物中采集肌肉和尾毛样本（每个处理 25 只动物）。肌肉中的（13）C/（12）C、（15）N/（14）N、（2）H/（1）H 和（34）S/（32）S 同位素比值清楚地反映了动物所摄入的饮食。通过应用逐步典范判别分析，可以根据饮食方案对牛肌肉进行很好的区分。基于分类成功率，肌肉中的（13）C/（12）C 和（34）S/（32）S 比值是鉴定来自不同饮食的牛肌肉的最佳指标。尾毛部分的（13）C/（12）C 和（15）N/（14）N 分析提供了宰前超过 1 年期间牛饮食变化的档案记录。
