Chemistry Department, Pharmaqam, Université du Québec à Montréal, Montréal, QC Canada.
J Agric Food Chem. 2011 Apr 13;59(7):3004-13. doi: 10.1021/jf105005q. Epub 2011 Mar 10.
Carotenoids have potent antioxidant activity as well as therapeutic value, and their formation has been seen to be induced in algae by stress, including high-salt culture conditions. A differential profiling of carotenoids was conducted using a targeted metabolomics approach with accurate mass data generated by liquid chromatography-electrospray-time-of-flight (LC-ESI-TOF) mass spectrometry followed by postacquisition filtering based on isotope patterns and mass defects to detect carotenoids up-regulated in Scenedesmus sp. exposed to high-salt conditions. Algal cultures treated with high concentrations of sodium acetate or sodium chloride were found to cause an increase in various carotenoids. On the basis of differential analysis, astaxanthin and canthaxanthin increased upon salt treatment. Astaxanthin, in its free form and as fatty acid esters, was seen to increase in Scenedesmus sp. using accurate mass MS. A few other carotenoid compounds increased upon salt treatment, including echinenone and adonirubin, involved in the pathway of astaxanthin biosynthesis from β-carotene, as well as isomers of astaxanthin and canthaxanthin. A time course study of acetate treatment was done to observe the time-dependent up-regulation of carotenogenesis.
类胡萝卜素具有很强的抗氧化活性和治疗价值，其在藻类中形成的过程被认为是受到应激的诱导，包括高盐培养条件。使用靶向代谢组学方法对类胡萝卜素进行了差异分析，该方法采用液相色谱-电喷雾-飞行时间（LC-ESI-TOF）质谱仪生成精确质量数据，并在采集后根据同位素模式和质量缺陷进行过滤，以检测暴露于高盐条件下的小球藻中上调的类胡萝卜素。研究发现，用高浓度醋酸钠或氯化钠处理藻类培养物会导致各种类胡萝卜素的增加。基于差异分析，盐处理后虾青素和角黄素增加。使用精确质量 MS 观察到小球藻中的游离形式和脂肪酸酯形式的虾青素增加。盐处理后还观察到一些其他类胡萝卜素化合物的增加，包括参与β-胡萝卜素向虾青素生物合成途径的echinenone 和adonirubin，以及虾青素和角黄素的异构体。对醋酸盐处理进行了时间过程研究，以观察类胡萝卜素生物合成的时间依赖性上调。
