石榴全果提取物对阿霉素诱导的大鼠毒性的心脏保护作用。
Cardioprotective effect of whole fruit extract of pomegranate on doxorubicin-induced toxicity in rat.
机构信息
Department of Pharmacology, Birjand University of Medical Sciences, Birjand, Iran.
出版信息
Pharm Biol. 2011 Apr;49(4):377-82. doi: 10.3109/13880209.2010.517758. Epub 2011 Mar 11.
CONTEXT
Cardioprotective effects of various plants are generally attributed to their antioxidant activity. The whole fruit extract of pomegranate (WFEP), Punica granatum L. (Punicaceae), has a potent antioxidant activity.
OBJECTIVE
To investigate cardioprotective effect of WFEP against doxorubicin (Dox)-induced cardiotoxicity in rats.
MATERIALS AND METHODS
Male Wistar rats were divided randomly into three groups of eight rats each: control (water, 5 mL/kg); Dox (10 mg/kg i.v.) and WFEP (100 mg/kg). Dox was administered in Dox and WFEP groups. After anesthetizing the animals on the last day, electrocardiogram was recorded and blood was analyzed for creatine kinase-MB isoenzyme (CK-MB), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) activities. Determinations of superoxide dismutase (SOD), reduced glutathione (GSH), lipid peroxidation (LPO) and histopathology of the heart tissues were carried out.
RESULTS
The WFEP group showed decreased QT and increase in heart rate (p < 0.05) compared to the Dox group. Significant decrease in CK-MB (p < 0.01), LDH (p < 0.05) and no such significant decrease in AST were observed as compared to the Dox group. There was significant increase in the level of GSH (p < 0.05), whereas inhibition of LPO and increase in SOD concentration was not significant in the WFEP group compared to the Dox group. Histopathological study of the WFEP-treated group showed slight protection against myocardial toxicity induced by Dox.
CONCLUSION
Results indicate that WFEP has cardioprotective effect against Dox-induced cardiotoxicity in rats.
背景
各种植物的心脏保护作用通常归因于其抗氧化活性。石榴全果提取物（WFEP），石榴（Punicaceae），具有很强的抗氧化活性。
目的
研究 WFEP 对阿霉素（Dox）诱导的大鼠心脏毒性的心脏保护作用。
材料和方法
雄性 Wistar 大鼠随机分为三组，每组 8 只：对照组（水，5 mL/kg）；Dox（10 mg/kg，iv）和 WFEP（100 mg/kg）。Dox 在 Dox 和 WFEP 组中给药。在最后一天麻醉动物后，记录心电图并分析血液中的肌酸激酶同工酶（CK-MB）、乳酸脱氢酶（LDH）和天冬氨酸氨基转移酶（AST）活性。测定超氧化物歧化酶（SOD）、还原型谷胱甘肽（GSH）、脂质过氧化（LPO）和心脏组织病理学。
结果
与 Dox 组相比，WFEP 组的 QT 缩短，心率增加（p < 0.05）。与 Dox 组相比，CK-MB（p < 0.01）和 LDH（p < 0.05）显著降低，AST 无明显降低。与 Dox 组相比，GSH 水平显著升高（p < 0.05），而 LPO 抑制和 SOD 浓度增加不显著。与 Dox 组相比，WFEP 治疗组的组织病理学研究显示对 Dox 诱导的心肌毒性有轻微保护作用。
结论
结果表明 WFEP 对大鼠 Dox 诱导的心脏毒性具有心脏保护作用。
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