Ahangarpour Akram, Heidari Razieh, Abdolahzadeh Mahsa, Oroojan Ali Akbar
Department of Physiology, Physiology and Diabetes Research Centers, Jundishapur University of Medical Sciences, Ahvaz, Iran.
J Reprod Infertil. 2012 Jul;13(3):138-42.
Punica granatum Linn. (PG) is native to the Mediterranean region. Its flower exhibited antioxidant activity. The present study attempt to investigate the effect of these extract on uterine contraction and its possible mechanism(s).
Thirty five female Wistar rats (200-300 g) at estrous phases of cycle was examined in this study; pieces of virgin adult rat uterus (1.5 cm) were suspended in an organ bath containing 10 ml of De Jalon solution at 29 °C. Tissue contractility was isometrically recorded. KCl (60 mM), BaCl2 (4 mM) and oxytocin (10 mU/ml) were applied to the tissue in the presence and absence of aqueous and hydroalcoholic extracts of the plant (0.05, 0.1, 0.2, 0.4 and 0.8 mg/ml). Propranolol (1 µM) and naloxane (1 µM) were added in KCl induced contractions. The results were analyzed by ANOVA and p < 0.05 were considered as significant.
Cumulative concentration of extracts reduced uterine contractions induced by KCl dose-dependently (p < 0.01). Extracts in a dose dependent (p < 0.05) reduced uterine contractions decreased dose-dependently after of addition oxytocin. The extracts added cumulatively to the organ bath reduced contractions but they did not affect uterine contractions induced by BaCl2 except the last dose. Spasmolytic effects of the extracts were not affected by propranolol or naloxane in KCl induced contractions.
Extracts diminished K(+)-induced contraction in uterus, therefore it seems that substances that decrease K(+)-induced contraction can also block voltage dependent calcium channel. The extracts did not have any effect on β-adrenoceptors or potassium channels.
石榴（Punica granatum Linn.，PG）原产于地中海地区。其花具有抗氧化活性。本研究旨在探讨这些提取物对子宫收缩的影响及其可能机制。
本研究选用35只处于动情周期的雌性Wistar大鼠（体重200 - 300克）；将成年未孕大鼠的子宫组织块（1.5厘米）悬挂于29℃含有10毫升德贾隆溶液的器官浴槽中。采用等长记录法记录组织收缩性。在有和没有该植物水提取物及水醇提取物（0.05、0.1、0.2、0.4和0.8毫克/毫升）存在的情况下，向组织施加氯化钾（60毫摩尔）、氯化钡（4毫摩尔）和催产素（10毫单位/毫升）。在氯化钾诱导的收缩实验中加入普萘洛尔（1微摩尔）和纳洛酮（1微摩尔）。结果采用方差分析进行分析，p < 0.05被认为具有统计学意义。
提取物的累积浓度剂量依赖性地降低了氯化钾诱导的子宫收缩（p < 0.01）。提取物剂量依赖性地（p < 0.05）降低了催产素加入后剂量依赖性降低的子宫收缩。向器官浴槽中累积加入提取物可降低收缩，但除最后一个剂量外，它们对氯化钡诱导的子宫收缩没有影响。在氯化钾诱导的收缩实验中，提取物的解痉作用不受普萘洛尔或纳洛酮的影响。
提取物减弱了子宫中钾离子诱导的收缩，因此似乎降低钾离子诱导收缩的物质也能阻断电压依赖性钙通道。提取物对β - 肾上腺素能受体或钾通道没有任何影响。
