Hemenway C, Weiss J, O'Connell K, Tumer N E
Monsanto Company, St. Louis, Missouri 63198.
Virology. 1990 Apr;175(2):365-71. doi: 10.1016/0042-6822(90)90421-m.
A full-length cDNA clone of potato virus X (PVX) has been constructed and fused to the bacteriophage T7 promoter in an in vitro transcription vector. Transcripts derived from this template (pMON 8660) were infectious when inoculated onto the local lesion host, Chenopodium amaranticolor. The infectivity of these transcripts was approximately 0.2% that of authentic PVX RNA. Lesions sampled from plants inoculated with these transcripts contained virus particles and virus aggregates typically observed in lesions from plants inoculated with authentic PVX RNA, as evidenced by electron microscopy. In addition, progeny virus isolated from these lesions was as infectious as progeny virus from an authentic PVX RNA infection when inoculated onto new local lesions plants. Infectious transcripts derived from PVX cDNA clones will facilitate analysis of the molecular aspects of PVX infection.
已构建马铃薯X病毒（PVX）的全长cDNA克隆，并将其与体外转录载体中的噬菌体T7启动子融合。当将源自该模板（pMON 8660）的转录本接种到局部病斑寄主苋色藜上时具有感染性。这些转录本的感染性约为真实PVX RNA的0.2%。通过电子显微镜观察证明，从接种这些转录本的植物上取样的病斑含有病毒粒子和病毒聚集体，这是接种真实PVX RNA的植物病斑中通常观察到的。此外，从这些病斑中分离出的子代病毒接种到新的局部病斑植物上时，其感染性与真实PVX RNA感染产生的子代病毒相同。源自PVX cDNA克隆的感染性转录本将有助于分析PVX感染的分子层面。
