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从全长克隆的cDNA合成感染性RNA至大花蕙兰环斑番茄病毒RNA

Synthesis of infectious RNA from full-length cloned cDNA to RNA of cymbidium ringspot tombusvirus.

作者信息

Burgyan J, Nagy P D, Russo M

机构信息

Dipartimento di Patologia Vegetale, Università degli Studi, Bari, Italy.

出版信息

J Gen Virol. 1990 Aug;71 ( Pt 8):1857-60. doi: 10.1099/0022-1317-71-8-1857.

DOI:10.1099/0022-1317-71-8-1857
PMID:1697330
Abstract

A full-length DNA copy of cymbidium ringspot virus RNA was cloned downstream of a phage T7 promoter. In vitro transcripts had no extra nucleotides at the 3' terminus, and a 5' end likely to be precisely as in genomic RNA. Transcripts were infective when inoculated into test plants. Northern blots from inoculated plants revealed the presence of genomic and subgenomic RNAs, but not of satellite RNA. Virus particles isolated from infected plants had the same outward aspect and size as those of the wild-type virus and were decorated by an antiserum to CyRSV in immune electron microscopy tests.

摘要

将大花蕙兰环斑病毒RNA的全长DNA拷贝克隆到噬菌体T7启动子下游。体外转录本在3'末端没有额外的核苷酸，5'末端可能与基因组RNA完全相同。接种到试验植物中的转录本具有感染性。接种植物的Northern杂交显示存在基因组RNA和亚基因组RNA，但不存在卫星RNA。从受感染植物中分离出的病毒粒子在外观和大小上与野生型病毒相同，并且在免疫电子显微镜试验中被抗CyRSV抗血清标记。

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