Infertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Hospital, Petach Tikva and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
Fertil Steril. 2011 May;95(6):2056-62. doi: 10.1016/j.fertnstert.2011.02.012. Epub 2011 Mar 10.
To investigate the expression of neurotrophin 3 (NT3) and its receptor tropomyosin-related kinase C (TrkC) in human preantral follicles. Neurotrophins appear to play important roles in preantral follicles. Data on ovarian NT3 and its receptor TrkC are sparse in humans.
Immunohistochemical, in situ hybridization, and reverse transcriptase polymerase chain reaction study of the expression of the NT3 system in human ovaries.
Major tertiary-care academic center.
PATIENT(S): Fifteen patients who underwent pregnancy terminations and 36 girls and women who underwent laparoscopies for ovarian surgery.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Laboratory analysis of human ovaries.
RESULT(S): NT3 protein staining was identified in oocytes and granulosa cells (GCs) of all samples tested. TrkC protein staining was present in oocytes and GCs of the majority of the fetal samples and in oocytes and GCs of all samples from girls and women. The messenger RNA transcripts for the full-length TrkC isoform were identified in oocytes of the majority of the fetal samples, in all the samples from the girls and women, and in GCs from the girls and women. The two NT3 isoforms and the three TrkC isoforms were identified by reverse transcription polymerase chain reaction in all ovarian extracts.
CONCLUSION(S): The presence of NT3 and its TrkC receptor in human preantral follicles, and specifically in GCs, suggests that NT3 may be involved in early folliculogenesis, particularly in the activation of primordial follicles.
研究神经营养因子 3（NT3）及其受体原肌球蛋白相关激酶 C（TrkC）在人原始卵泡中的表达。神经营养因子在原始卵泡中似乎起着重要作用。关于卵巢 NT3 及其受体 TrkC 的数据在人类中很少。
对人卵巢中 NT3 系统的表达进行免疫组织化学、原位杂交和逆转录聚合酶链反应研究。
主要的三级保健学术中心。
15 名因妊娠终止而行手术的患者和 36 名因卵巢手术而行腹腔镜检查的女孩和妇女。
无。
人卵巢的实验室分析。
在所有检测的样本中，均发现 NT3 蛋白染色存在于卵母细胞和颗粒细胞（GC）中。TrkC 蛋白染色存在于大多数胎儿样本的卵母细胞和 GC 中，以及所有女孩和妇女样本的卵母细胞和 GC 中。全长 TrkC 异构体的信使 RNA 转录本在大多数胎儿样本的卵母细胞中、所有女孩和妇女的样本中以及女孩和妇女的 GC 中均被鉴定出来。通过逆转录聚合酶链反应，在所有卵巢提取物中均鉴定出两种 NT3 异构体和三种 TrkC 异构体。
NT3 及其 TrkC 受体存在于人原始卵泡中，特别是在 GC 中，这表明 NT3 可能参与早期卵泡发生，特别是原始卵泡的激活。
