Department of Prosthodontics and Dental Research Institute, School of Dentistry, Seoul National University, 275-1 Yeongeon-Dong Jongno-Gu, Seoul 110-768, Republic of Korea.
J Dent Res. 2011 Jun;90(6):711-6. doi: 10.1177/0022034511400074. Epub 2011 Mar 10.
Surface energy and hydrophilicity of implant surfaces have been known to play an important role in subsequent cellular responses on the implant surface. The aim of the present study was to evaluate the effects of biomimetic deposition of anodized surfaces on surface wettability, surface energy, and osteoblast responses. Ti discs with 2 different surface topographies (machined and anodized) were immersed in Hanks' balanced salt solution (HBSS) and modified simulated body fluid (SBF) solution for 2 weeks at physiologic conditions of 37 °C, initial pH of 7.4, and p(CO(2)) of 0.05 atm. Scanning electron microscopic (SEM) observation and energy-dispersive spectroscopic (EDS) microanalysis showed the deposition of calcium phosphate (CaP) onto anodized Ti surfaces immersed in modified SBF. Surface energy, surface wettability, and osteoblast responses, including cell attachment capacity, cell proliferation rate, and cell differentiation level, significantly increased on anodized Ti surfaces immersed in modified SBF. The effects of biomimetic deposition with modified SBF on physiochemical surface characteristics and cell biological responses were greater on anodized surfaces than on machined surfaces. These results indicate that biomimetic deposition with effective SBF may enhance the interaction between anodized Ti surfaces and their biological environment, consequently improving bone healing of dental Ti implants.
种植体表面的表面能和亲水性已被证明在种植体表面随后的细胞反应中起着重要作用。本研究旨在评估仿生沉积氧化表面对表面润湿性、表面能和成骨细胞反应的影响。将具有 2 种不同表面形貌（机械加工和阳极氧化）的 Ti 圆盘在生理条件下（37°C、初始 pH 值为 7.4 和 p(CO(2))为 0.05 atm）在 Hank's 平衡盐溶液 (HBSS) 和改良模拟体液 (SBF) 溶液中浸泡 2 周。扫描电子显微镜 (SEM) 观察和能谱 (EDS) 微分析表明，浸入改良 SBF 的阳极氧化 Ti 表面上沉积了钙磷 (CaP)。浸入改良 SBF 的阳极氧化 Ti 表面的表面能、表面润湿性和成骨细胞反应（包括细胞黏附能力、细胞增殖率和细胞分化水平）显著增加。与机械加工表面相比，仿生沉积与改良 SBF 对理化表面特性和细胞生物学反应的影响在阳极氧化表面上更大。这些结果表明，有效的 SBF 仿生沉积可以增强阳极氧化 Ti 表面与其生物环境之间的相互作用，从而改善牙科 Ti 种植体的骨愈合。
