HemoLeon, Grupo Cardiovascular-Fundación Investigación Sanitaria en León, Hospital de León, e Instituto de Biomedicina IBIOMED, Universidad de León, León, Spain.
Vet Pathol. 2011 Nov;48(6):1109-17. doi: 10.1177/0300985811400446. Epub 2011 Mar 10.
Late thrombosis of coronary drug-eluting stents is an infrequent but serious complication of percutaneous transluminal coronary angioplasty. The best predictor of this event is the lack of endothelialization of stent struts. The objective of this study is to characterize and quantify the time course of endothelialization of different stents implanted in nonatherosclerotic swine coronary arteries. Thirty-three Carbofilm-coated stents were implanted percutaneously in 11 anesthetized domestic, crossbred pigs (weight 25 ± 3 kg, 2 months old). Each animal received 1 stainless steel stent (SS), 1 cobalt-chromium stent (CCS), and 1 tacrolimus-eluting stent (TES) in each coronary artery. Follow-up periods were 1 day (n = 9 stents), 3 days (n = 9 stents), and 7 days (n = 15 stents). Longitudinal sections of the stented vessels were examined using scanning electron microscopy. At 1 day, there was scarce, patchy endothelialization with areas of fibrin; the endothelialization rate was similar for all the stents (SS, 29% ± 23%; CCS, 29% ± 24%; TES, 31% ± 25%; P = .9). At 3 days, there were more endothelial cells but with immature features and giant cells over fibrin; the endothelialization was greater in SS and CCS than in TES (SS, 79% ± 14%; CCS, 81% ± 17%; TES, 46% ± 9%; P = .007). At 7 days, arteries showed better endothelialization with few giant cells; the endothelialization was greater in SS and CCS than in TES (SS, 95% ± 4%; CCS, 98% ± 4%; TES, 79% ± 9%; P = .01). In conclusion, the described model is useful for the analysis of endothelialization of coronary stents and facilitates measurement of its rate of formation and characterization of the involved cell types.
经皮腔内冠状动脉血管成形术后晚期冠状动脉药物洗脱支架血栓形成是一种罕见但严重的并发症。该事件的最佳预测指标是支架支柱的内皮化缺失。本研究的目的是描述和量化不同支架在非动脉粥样硬化猪冠状动脉内的内皮化时间过程。将 33 个 Carbofilm 涂层支架经皮植入 11 只麻醉的杂种猪（体重 25 ± 3kg，2 个月龄）的 11 条冠状动脉内。每只动物在每条冠状动脉内分别植入 1 个不锈钢支架（SS）、1 个钴铬支架（CCS）和 1 个他克莫司洗脱支架（TES）。随访期为 1 天（n = 9 个支架）、3 天（n = 9 个支架）和 7 天（n = 15 个支架）。使用扫描电子显微镜对支架血管的纵向切片进行检查。在 1 天，有少量、斑驳的内皮化，伴有纤维蛋白区；所有支架的内皮化率相似（SS，29% ± 23%；CCS，29% ± 24%；TES，31% ± 25%；P =.9）。在 3 天，有更多的内皮细胞，但具有不成熟的特征和纤维蛋白上的巨细胞；SS 和 CCS 的内皮化大于 TES（SS，79% ± 14%；CCS，81% ± 17%；TES，46% ± 9%；P =.007）。在 7 天，动脉显示出更好的内皮化，只有少数巨细胞；SS 和 CCS 的内皮化大于 TES（SS，95% ± 4%；CCS，98% ± 4%；TES，79% ± 9%；P =.01）。总之，该模型可用于分析冠状动脉支架的内皮化，并有助于测量其形成速度和所涉及的细胞类型的特征。
