金黄色葡萄球菌MazF mRNA干扰酶的结晶
Crystallization of the Staphylococcus aureus MazF mRNA interferase.
作者信息
Zorzini Valentina, Haesaerts Sarah, Donegan Niles P, Fu Zhibiao, Cheung Ambrose L, van Nuland Nico A J, Loris Remy
机构信息
Structural Biology Brussels, Department of Molecular and Cellular Interactions, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel, Belgium.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Mar 1;67(Pt 3):386-9. doi: 10.1107/S1744309111000571. Epub 2011 Feb 25.
Abstract
mazEF modules encode toxin-antitoxin pairs that are involved in the bacterial stress response through controlled and specific degradation of mRNA. Staphylococcus aureus MazF and MazE constitute a unique toxin-antitoxin module under regulation of the sigB operon. A MazF-type mRNA interferase is combined with an antitoxin of unknown fold. Crystals of S. aureus MazF (SaMazF) were grown in space group P2(1)2(1)2(1). The crystals diffracted to 2.1 Å resolution and are likely to contain two SaMazF dimers in the asymmetric unit.
摘要
mazEF模块编码毒素-抗毒素对，这些对通过对mRNA的可控且特异性降解参与细菌应激反应。金黄色葡萄球菌的MazF和MazE在sigB操纵子的调控下构成一个独特的毒素-抗毒素模块。一种MazF型mRNA干扰酶与一种折叠未知的抗毒素结合。金黄色葡萄球菌MazF（SaMazF）的晶体在空间群P2(1)2(1)2(1)中生长。这些晶体衍射到2.1 Å分辨率，并且在不对称单元中可能包含两个SaMazF二聚体。
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