Antimicrobial Resistance Unit, Mycobacterial Laboratory, National Institute for Health and Welfare, Kiinamyllynkatu 13, 20520 Turku, Finland.
Eur J Clin Microbiol Infect Dis. 2011 Sep;30(9):1119-26. doi: 10.1007/s10096-011-1200-7. Epub 2011 Mar 11.
Drug resistance and molecular epidemiology of tuberculosis (TB) in the Murmansk region was investigated in a 2-year, population-based surveillance of the civilian population. During 2003 and 2004, isolates from all culture-positive cases were collected (n = 1,226). Prevalence of multi-drug resistance (MDR) was extremely high, as 114 out of 439 new cases (26.0%), and 574 out of 787 previously treated cases (72.9%) were resistant to at least isoniazid (INH) and rifampin (RIF). Spoligotyping of the primary MDR-TB isolates revealed that most isolates grouped to the Beijing SIT1 genotype (n = 91, 79.8%). Isolates of this genotype were further analyzed by IS6110 RFLP. Sequencing of gene targets associated with INH and RIF resistance further showed that the MDR-TB strains are highly homogeneous as 78% of the MDR, SIT1 strains had the same resistance-conferring mutations. The genetic homogeneity of the MDR-TB strains indicates that they are actively transmitted in Murmansk.
在对摩尔曼斯克地区的平民进行的为期两年的基于人群的监测中，研究了结核病（TB）的耐药性和分子流行病学。在 2003 年和 2004 年，收集了所有培养阳性病例的分离株（n = 1,226）。耐多药（MDR）的流行率极高，因为 439 例新发病例中有 114 例（26.0％），787 例既往治疗病例中有 574 例（72.9％）对至少异烟肼（INH）和利福平（RIF）具有耐药性。主要 MDR-TB 分离株的 spoligotyping 显示，大多数分离株属于北京 SIT1 基因型（n = 91，79.8％）。进一步对这种基因型的分离株进行了 IS6110 RFLP 分析。与 INH 和 RIF 耐药相关的基因靶标测序进一步表明，MDR-TB 菌株高度同源，因为 78％的 MDR，SIT1 菌株具有相同的耐药性突变。MDR-TB 菌株的遗传同质性表明它们在摩尔曼斯克地区正在积极传播。
