Department of Environmental Sciences, University of Siena, 53100, Siena, Italy.
Folia Microbiol (Praha). 2011 Jan;56(1):29-35. doi: 10.1007/s12223-011-0010-8. Epub 2011 Mar 11.
Five arsenic-resistant bacterial strains (designated MP1400, MP1400a, MP1400d, APSLA3, and BPSLA3) were isolated from soils collected at the Alps region (Italy), which showed no contamination by arsenic. Phylogenetic analysis of the 16S rRNA gene sequences assigned them to the genera Pseudomonas and Bacillus. Bacillus sp. strain 1400d and Pseudomonas spp. strains APSLA3 and MP1400 showed higher tolerance to As(III), as indicated by minimum inhibitory concentrations of 10 mmol/L. Pseudomonas sp. strain MP1400 exhibited higher tolerance to As(V) (minimum inhibitory concentration of 135 mmol/L). The isolated arsenic-resistant strains were able to reduce As(V) to As(III), especially Pseudomonas sp. strain MP1400 reducing 2 mmol/L of As(V) to As(III) within 24 h. The results suggest that the isolated bacterial strains play a role in the arsenic biogeochemical cycle of arsenic-poor soils in the Alps mount area.
从意大利阿尔卑斯山地区采集的未受砷污染的土壤中分离到 5 株砷抗性细菌（命名为 MP1400、MP1400a、MP1400d、APSLA3 和 BPSLA3）。16S rRNA 基因序列的系统发育分析将它们分配到假单胞菌属和芽孢杆菌属。芽孢杆菌属 1400d 菌株和假单胞菌属菌株 APSLA3 和 MP1400 对 As(III)的耐受性更高，最低抑菌浓度为 10 mmol/L。假单胞菌属菌株 MP1400 对 As(V)的耐受性更高（最低抑菌浓度为 135 mmol/L）。分离出的砷抗性菌株能够将 As(V)还原为 As(III)，特别是假单胞菌属菌株 MP1400 在 24 小时内将 2 mmol/L 的 As(V)还原为 As(III)。结果表明，这些分离的细菌菌株在阿尔卑斯山贫砷土壤的砷生物地球化学循环中发挥作用。
