Shivaji S, Suresh K, Chaturvedi Preeti, Dube Smita, Sengupta S
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Geological Survey of India, GSI Complex, DK-6, Sector-II, Bidhannagar, Kolkata - 700 091, India.
Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1123-1127. doi: 10.1099/ijs.0.63476-0.
Strain Con a/3(T) is a Gram-positive, motile, endospore-forming, rod-shaped and arsenic-resistant bacterium, which was isolated from a concretion of arsenic ore obtained from a bore-hole. The bacterium grew in the presence of 20 mM arsenate and 0.5 mM arsenite. Diaminopimelic acid was present in the cell wall peptidoglycan, MK-7 was the major menaquinone, and iso-C(15 : 0), anteiso-C(15 : 0), iso-C(16 : 0) and C(16 : 1)(delta7cis) were the major fatty acids. Based on its phenotypic, chemotaxonomic and phylogenetic characteristics, strain Con a/3(T) was identified as a member of the genus Bacillus. It exhibited maximum similarity (97 %) at the 16S rRNA gene level with Bacillus barbaricus (DSM 14730(T)); however, the DNA-DNA relatedness value with B. barbaricus was 60 %. Strain Con a/3(T) also exhibited a number of phenotypic differences from B. barbaricus (DSM 14730(T)). Strain Con a/3(T) was therefore identified as representing a novel species of the genus Bacillus, for which the name Bacillus arsenicus sp. nov. is proposed. The type strain is Con a/3(T) (= MTCC 4380(T) = DSM 15822(T) = JCM 12167(T)).
Con a/3(T)菌株是一种革兰氏阳性、具运动性、产芽孢、杆状且抗砷的细菌，它是从一个钻孔获得的砷矿石结核中分离出来的。该细菌能在20 mM砷酸盐和0.5 mM亚砷酸盐存在的情况下生长。细胞壁肽聚糖中存在二氨基庚二酸，MK-7是主要的甲基萘醌，异-C(15 : 0)、anteiso-C(15 : 0)、异-C(16 : 0)和C(十六碳烯酸)(δ7顺式)是主要脂肪酸。基于其表型、化学分类和系统发育特征，Con a/3(T)菌株被鉴定为芽孢杆菌属的一个成员。它在基于16S rRNA基因水平上与巴氏芽孢杆菌(DSM 14730(T))表现出最大相似性(97%)；然而，与巴氏芽孢杆菌的DNA-DNA相关性值为60%。Con a/3(T)菌株也表现出与巴氏芽孢杆菌(DSM 14730(T))的一些表型差异。因此，Con a/3(T)菌株被鉴定为代表芽孢杆菌属的一个新物种，为此提出名称为砷芽孢杆菌（Bacillus arsenicus）新种。模式菌株是Con a/3(T)(= MTCC 4380(T) = DSM 15822(T) = JCM 12167(T))。
