Milam A H
Scheie Eye Institute, Department of Ophthalmology, University of Pennsylvania Health System, Philadelphia, PA.
Methods Mol Med. 2001;47:71-88. doi: 10.1385/1-59259-085-3:71.
It is often desirable to localize a specific protein within a layer, cell, or subcellular organelle in the retina. A variety of immunocytochemical techniques are available to achieve this goal. These techniques all utilize an antibody that binds to the protein of interest and is detected by a label (a fluorescent dye, enzyme, or electron dense marker) to localize the specific antigen in the tissue.
通常希望在视网膜的某一层、细胞或亚细胞器内定位特定蛋白质。有多种免疫细胞化学技术可实现这一目标。这些技术都利用一种与感兴趣的蛋白质结合的抗体，并通过标记物（荧光染料、酶或电子致密标记物）进行检测，以在组织中定位特定抗原。
