Pfeiffer B, Grosche J, Reichenbach A, Hamprecht B
Physiologisch-Chemisches Institut, University of Tübingen, Germany.
Glia. 1994 Sep;12(1):62-7. doi: 10.1002/glia.440120108.
Glycogen phosphorylase (GP) was immunocytochemically detected in Müller cells of the rabbit and rat retina using a monoclonal antibody raised against bovine brain GP. Immunofluorescence and immunoenzymatic procedure were applied on isolated, Müller cells and sections of paraformaldehyde-fixed, paraffin-embedded retinas. All methods used revealed positive immunostaining. GP immunoreactivity was most intense in the Müller cell endfeet and the pericarya, corresponding to the nerve fibre layer and the inner nuclear layer in the retina. The presence of GP in Müller cells stresses the important role of these glial cells in the energy metabolism of the mammalian retina.
使用针对牛脑糖原磷酸化酶（GP）产生的单克隆抗体，通过免疫细胞化学方法在兔和大鼠视网膜的 Müller 细胞中检测到了 GP。对分离的 Müller 细胞以及经多聚甲醛固定、石蜡包埋的视网膜切片进行了免疫荧光和免疫酶法检测。所有使用的方法均显示出阳性免疫染色。GP 的免疫反应性在 Müller 细胞终足和胞体中最为强烈，分别对应于视网膜中的神经纤维层和内核层。Müller 细胞中 GP 的存在强调了这些神经胶质细胞在哺乳动物视网膜能量代谢中的重要作用。
