Department of Materials Chemistry, Uppsala University, SE-751 21 Uppsala, Sweden.
J Tissue Eng Regen Med. 2011 Aug;5(8):e188-96. doi: 10.1002/term.415. Epub 2011 Mar 10.
The present work describes the feasibility of a cross-linkable injectable hyaluronan hydrogel for cartilage repair. The hydrogel used is a two-component system based on aldehyde-modified hyaluronan and hydrazide-modified polyvinyl alcohol, which are rapidly cross-linked in situ upon mixing. The in vitro study showed that chondrocytes and mesenchymal cells cultured in the gel form cartilage-like tissue, rich in glycosaminoglycans, collagen type II and aggrecan. In a rabbit animal model the injection of the hydrogel improved the healing of a full-thickness cartilage defect created in the knee as compared to non-treated controls. This rabbit study showed that the regenerated cartilage defects stained more intensely for type II collagen upon treatment with the hydrogel. The hyaluronan-based hydrogel may be used as a delivery vehicle for both growth factors and/or cells for cartilage repair. The in vivo study also indicated that the hydrogel alone has a beneficial effect on cartilage regeneration.
本研究描述了一种可交联的透明质酸水凝胶在软骨修复中的可行性。所使用的水凝胶是一种基于醛基修饰透明质酸和酰肼修饰聚乙烯醇的双组分体系，混合后可迅速原位交联。体外研究表明，培养在凝胶中的软骨细胞和成纤维细胞可形成富含糖胺聚糖、Ⅱ型胶原和聚集蛋白聚糖的软骨样组织。在兔动物模型中，与未经处理的对照组相比，膝关节全层软骨缺损注射水凝胶后可改善愈合。该兔研究表明，与未经处理的对照组相比，用该水凝胶处理后的再生软骨缺陷中Ⅱ型胶原染色更深。基于透明质酸的水凝胶可用作软骨修复的生长因子和/或细胞的递送载体。体内研究还表明，水凝胶本身对软骨再生具有有益作用。
