Wang Yanfei, Zhao Hongchun, Chen Jun, Liu Lianxin, Zhang Peng, Che Juan, Wang Geng
Department of Otolaryngology Head and Neck Surgery, the Affiliated Hospital of Binzhou Medical College, Binzhou, 256603, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2010 Dec;24(24):1112-5.
To investigate the role and clinical value of the serum level of hepatocyte growth factor (HGF) and vascular endothelial growth factor(VEGF) in laryngeal squamous cell cancer and the relationship between HGF, VEGF and clinicopathological factors.
We measured serum HGF and VEGF level in 54 laryngeal squamous cell cancer patients using ELISA to demonstrate the variation of HGF, VEGF level before operation and at day 1, 3, 7, 14 post-surgery. The concentration of the serum HGF, VEGF content in 35 normal healthy people and in 30 vocal cords polyps patients were measured by the quantitative sandwich enzyme linked immunoassay technique.
(1) The level of serum HGF and VEGF in the patients with laryngeal squamous cell cancer was significantly higher than that of normal healthy people and vocal cord polyps patients (P < 0.01). (2) The level of serum HGF, VEGF in the patients with laryngeal squamous cell cancer was related to clinical stage, differentiation situation, lymph nodes metastasis (P < 0.01), but it was independent of age at diagnosis, gender and tumor location (P > 0.05). (3) There was a significantly positive correlation between preoperative serum VEGF and HGF levels (r = 0.7667, P < 0.01). (4) Post-operational serum HGF levels in 54 laryngeal squamous cell cancer patients who underwent surgical intervention increased significantly, peaked at day 3 after operation. Serum HGF levels of survivors during follow-up period gradually decreased at day 7 and day 14 after operation. The postoperative serum levels of VEGF were decreased significantly than that of preoperation.
HGF and VEGF may play an important role in the development and progression of human laryngeal cancer. Elevated serum HGF and VEGF levels predict a more aggressive biological behavior in laryngeal squamous cell cancer.
探讨肝细胞生长因子（HGF）和血管内皮生长因子（VEGF）血清水平在喉鳞状细胞癌中的作用及临床价值，以及HGF、VEGF与临床病理因素之间的关系。
采用酶联免疫吸附测定法（ELISA）检测54例喉鳞状细胞癌患者血清HGF和VEGF水平，以观察手术前及术后第1、3、7、14天HGF、VEGF水平的变化。采用定量夹心酶联免疫测定技术检测35例正常健康人和30例声带息肉患者血清HGF、VEGF含量。
（1）喉鳞状细胞癌患者血清HGF和VEGF水平显著高于正常健康人和声带息肉患者（P<0.01）。（2）喉鳞状细胞癌患者血清HGF、VEGF水平与临床分期、分化程度、淋巴结转移有关（P<0.01），但与诊断时年龄、性别及肿瘤部位无关（P>0.05）。（3）术前血清VEGF与HGF水平呈显著正相关（r=0.7667，P<0.01）。（4）54例接受手术干预的喉鳞状细胞癌患者术后血清HGF水平显著升高，术后第3天达到峰值。随访期间存活患者术后第7天和第14天血清HGF水平逐渐下降。术后血清VEGF水平较术前显著降低。
HGF和VEGF可能在人类喉癌的发生发展中起重要作用。血清HGF和VEGF水平升高预示喉鳞状细胞癌具有更具侵袭性的生物学行为。
