Shang Z J, Li J R, Li Z B
Department of Oral and Maxillofacial Surgery, College and Hospital of Stomatology, Wuhan University, China.
Int J Oral Maxillofac Surg. 2002 Oct;31(5):495-8. doi: 10.1054/ijom.2002.0284.
Angiogenesis is a crucial step in tumour growth, progression, and metastasis. Recently, many angiogenic factors have been identified. Vascular endothelial growth factor (VEGF) is thought to be one such angiogenic factor and is also thought to be a selective mitogen for vascular endothelial cells. The present study was designed to determine the circulating levels of VEGF in human oral squamous cell carcinoma (OSCC) and to investigate its correlation with the clinicopathologic features and prognosis. The study consisted of 10 healthy volunteers and 31 patients with OSCC. A quantitative sandwich enzyme immunoassay technique was used to measure the concentrations of VEGF in the sera. The data obtained from the experiment were analysed with independent-samples t-test. The mean concentrations of serum VEGF were 148.80 +/- 64.17 pg x ml(-1) (range, 59-231 pg x ml(-1)) in normal controls and 567.97 +/- 338.17 pg x ml(-1) (range, 136-1,892 pg x ml(-1)) in OSCC group. Statistical analysis showed that the mean VEGF level in sera of OSCC patients was significantly higher than that in healthy controls (P<0.001). The serum VEGF levels of OSCC patients correlated with condition of nodal metastasis (P<0.05) and clinical stage (P<0.05) but not with sex (P>0.05) and degree of differentiation (P>0.05). Our study showed that OSCC was associated with significantly elevated serum VEGF concentration. Higher level of serum VEGF also correlated with lymph node metastasis and clinical stage of OSCC. Determination of serum VEGF concentration may be helpful to distinguish OSCC patients from the normal individuals.
血管生成是肿瘤生长、进展和转移的关键步骤。近年来，已鉴定出许多血管生成因子。血管内皮生长因子（VEGF）被认为是其中一种血管生成因子，也被认为是血管内皮细胞的选择性有丝分裂原。本研究旨在测定人类口腔鳞状细胞癌（OSCC）患者血清中VEGF的循环水平，并探讨其与临床病理特征及预后的相关性。该研究包括10名健康志愿者和31例OSCC患者。采用定量夹心酶免疫测定技术检测血清中VEGF的浓度。实验所得数据采用独立样本t检验进行分析。正常对照组血清VEGF的平均浓度为148.80±64.17 pg·ml⁻¹（范围为59 - 231 pg·ml⁻¹），OSCC组为567.97±338.17 pg·ml⁻¹（范围为136 - 1892 pg·ml⁻¹）。统计学分析表明，OSCC患者血清中VEGF的平均水平显著高于健康对照组（P<0.001）。OSCC患者血清VEGF水平与淋巴结转移情况（P<0.05）和临床分期（P<0.05）相关，但与性别（P>0.05）和分化程度（P>0.05）无关。我们的研究表明，OSCC与血清VEGF浓度显著升高有关。血清VEGF水平升高还与OSCC的淋巴结转移及临床分期相关。测定血清VEGF浓度可能有助于区分OSCC患者与正常个体。
