Nirala Satendra K, Li Peiqiang, Bhadauria Monika, Guo Guangqin
Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou, China Institute of Genetics, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China National Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, China.
Integr Zool. 2008 Sep;3(3):194-207. doi: 10.1111/j.1749-4877.2008.00090.x.
The combined effect of gallic acid (3,4,5-trihydroxy benzoic acid; GA; 50 mg kg(-1) i.p.) and propolis (200 mg kg(-1) p.o.) was evaluated against beryllium-induced biochemical and morphological alterations in the liver and kidney. Female albino rats were exposed to beryllium nitrate (1 mg kg(-1) i.p.) daily for 28 days followed by treatment with the above mentioned therapeutic agents either individually or in combination for five consecutive days. Exposure to beryllium increased its concentration in the serum, liver and kidney and caused significant alterations in cytochrome P450 enzymes, microsomal lipid peroxidation and protein contents. Beryllium administration significantly altered the aspartate aminotransaminase, alanine aminotransaminase, lactate dehydrogenase, γ-grutamy1 transpeptidase, bilirubin, creatinine and urea in serum, and the activity of acid phosphatase, alkaline phosphatase, adenosine triphosphatase, glucose-6-phophatase and succinic dehydrogenase, triglycerides, cholesterol, protein contents, glycogen contents, lipid peroxidation and glutathione level in the liver and kidney. Beryllium exposure induced severe alterations in hepatorenal morphology, revealing its toxic consequences at a cellular level. Individual administration of GA and propolis reduced the effects on the studied parameters to a degree. Interestingly, GA in conjunction with propolis reversed the alterations in all of the variables examined, highlighting the beneficial effects of combined therapy over monotherapy in the alleviation of beryllium-induced systemic toxicity.
评估了没食子酸（3,4,5 - 三羟基苯甲酸；GA；50毫克/千克腹腔注射）和蜂胶（200毫克/千克口服）联合使用对铍诱导的肝脏和肾脏生化及形态学改变的影响。雌性白化大鼠每天腹腔注射硝酸铍（1毫克/千克），持续28天，随后连续五天单独或联合使用上述治疗剂进行治疗。接触铍会增加其在血清、肝脏和肾脏中的浓度，并导致细胞色素P450酶、微粒体脂质过氧化和蛋白质含量发生显著改变。给予铍会显著改变血清中的天冬氨酸转氨酶、丙氨酸转氨酶、乳酸脱氢酶、γ - 谷氨酰转肽酶、胆红素、肌酐和尿素，以及肝脏和肾脏中酸性磷酸酶、碱性磷酸酶、三磷酸腺苷酶、葡萄糖 - 6 - 磷酸酶和琥珀酸脱氢酶的活性、甘油三酯、胆固醇、蛋白质含量、糖原含量、脂质过氧化和谷胱甘肽水平。铍暴露会引起肝肾形态的严重改变，揭示了其在细胞水平上的毒性后果。单独给予GA和蜂胶在一定程度上减轻了对所研究参数的影响。有趣的是，GA与蜂胶联合使用可逆转所有检测变量的改变，突出了联合治疗相对于单一治疗在减轻铍诱导的全身毒性方面的有益效果。
