Nirala Satendra Kumar, Bhadauria Monika, Mathur Ramesh, Mathur Asha
School of Studies in Zoology, Jiwaji University, Gwalior-474011, India.
J Appl Toxicol. 2008 Jan;28(1):44-54. doi: 10.1002/jat.1250.
The therapeutic potential of the chelator tiferron (sodium-4,5-dihydroxy-1,3-benzene disulphonate; 300 mg kg(-1), i.p.) and adjuvants, i.e. alpha-tocopherol (25 mg kg(-1), p.o.), propolis (a honey-bee hive product; 200 mg kg(-1), p.o.) and piperine (10 mg kg(-1), p.o.) were evaluated individually and in combination against beryllium induced biochemical alterations and oxidative stress consequences. Female albino rats were exposed to beryllium nitrate (1 mg kg(-1), i.p.) daily for 28 days followed by treatment with the above mentioned therapeutic agents for 5 consecutive days. Administration of beryllium altered blood biochemical variables with significant depletion in hemoglobin, blood sugar, total serum protein, albumin and significant enhancement in the release of serum transaminases. A significantly increased lipid peroxidation and a decreased level of glutathione after beryllium exposure indicated oxidative stress in the liver and kidney. Beryllium exposure decreased total protein and glycogen contents, whereas triglycerides and cholesterol increased significantly in liver and kidney. Individual administration of all the four compounds showed significant therapeutic potential in reverse of some of the biochemical parameters mentioned above. Furthermore, the combination of tiferron with alpha-tocopherol, propolis or piperine, respectively, could reverse all the variables significantly more towards the control. None of the test compounds showed any significant change in choleretic activity (bile flow and bile solids), indicating that these compounds had no adverse effects at these dose levels. It was concluded that all the combinations of tiferron and adjuvants played a beneficial role in reducing beryllium induced systemic toxicity at relatively lower doses and the combination of tiferron and propolis showed a more pronounced therapeutic potential.
评估了螯合剂替菲罗（4,5-二羟基-1,3-苯二磺酸钠；300毫克/千克，腹腔注射）以及佐剂，即α-生育酚（25毫克/千克，口服）、蜂胶（一种蜂巢产品；200毫克/千克，口服）和胡椒碱（10毫克/千克，口服）单独及联合使用对铍诱导的生化改变和氧化应激后果的治疗潜力。雌性白化大鼠每天腹腔注射硝酸铍（1毫克/千克），持续28天，随后连续5天用上述治疗药物进行治疗。铍的给药改变了血液生化指标，血红蛋白、血糖、总血清蛋白、白蛋白显著减少，血清转氨酶释放显著增加。铍暴露后脂质过氧化显著增加，谷胱甘肽水平降低，表明肝脏和肾脏存在氧化应激。铍暴露降低了肝脏和肾脏中的总蛋白和糖原含量，而甘油三酯和胆固醇显著增加。所有四种化合物单独给药均显示出对上述一些生化参数的逆转具有显著治疗潜力。此外，替菲罗分别与α-生育酚、蜂胶或胡椒碱联合使用，可使所有变量更显著地向对照值逆转。所有受试化合物的利胆活性（胆汁流量和胆汁固体）均未显示任何显著变化，表明这些化合物在这些剂量水平下无不良影响。得出的结论是，替菲罗与佐剂的所有组合在相对较低剂量下对降低铍诱导的全身毒性发挥了有益作用，且替菲罗与蜂胶的组合显示出更显著的治疗潜力。
