Dunn S D, Dallmann H G
Department of Biochemistry, University of Western Ontario, London, Canada.
J Bacteriol. 1990 May;172(5):2782-4. doi: 10.1128/jb.172.5.2782-2784.1990.
The effect of upstream uncD sequences on expression of the Escherichia coli uncC gene, encoding the epsilon subunit of F1-ATPase, was studied. uncC expression was reduced severalfold in plasmid constructs bearing, in addition to uncC, a region of uncD located between 85 and 119 bases upstream from the uncC initiation codon. This reduction was independent of in-frame translation of the uncD sequences. An mRNA stem-loop structure in which sequences located within the inhibitory region of uncD base pair with the uncDC intercistronic region is suggested to function in modulating uncC expression.
研究了上游uncD序列对编码F1 - ATP合酶ε亚基的大肠杆菌uncC基因表达的影响。在除uncC之外还带有uncD区域（位于uncC起始密码子上游85至119个碱基之间）的质粒构建体中，uncC表达降低了数倍。这种降低与uncD序列的读码框内翻译无关。有人提出，一种mRNA茎环结构在调节uncC表达中起作用，在该结构中，位于uncD抑制区域内的序列与uncDC基因间区域形成碱基对。
