McCarthy J E, Gerstel B, Surin B, Wiedemann U, Ziemke P
Department of Gene Expression, GBF-Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany.
Mol Microbiol. 1991 Oct;5(10):2447-58. doi: 10.1111/j.1365-2958.1991.tb02090.x.
The atp operon of Escherichia coli directs synthesis rates of protein subunits that are well matched to the requirements of assembly of the membrane-bound H(+)-ATPase (alpha 3 beta 3 gamma 1 delta 1 epsilon 1a1b2c10-15). Segmental differences in mRNA stability are shown to contribute to the differential control of atp gene expression. The first two genes of the operon, atpl and atpB, are rapidly inactivated at the mRNA level. The remaining seven genes are more stable. It has previously been established that the translational efficiencies of the atp genes vary greatly. Thus differential expression from this operon is achieved via post-transcriptional control exerted at two levels. Neither enhancement of translational efficiency nor insertion of repetitive extragenic palindromic (REP) sequences into the atplB intercistronic region stabilized atpl. We discuss the implications of these results in terms of the pathway of mRNA degradation and of the role of mRNA stability in the control of gene expression.
大肠杆菌的atp操纵子指导蛋白质亚基的合成速率，这些亚基与膜结合的H(+)-ATP酶（α3β3γ1δ1ε1a1b2c10 - 15）组装的需求高度匹配。mRNA稳定性的片段差异被证明有助于atp基因表达的差异控制。操纵子的前两个基因atpl和atpB在mRNA水平迅速失活。其余七个基因更稳定。先前已经确定，atp基因的翻译效率差异很大。因此，该操纵子的差异表达是通过在两个水平上施加的转录后控制实现的。提高翻译效率或在atplB基因间区域插入重复的基因外回文（REP）序列都不能使atpl稳定。我们根据mRNA降解途径以及mRNA稳定性在基因表达控制中的作用来讨论这些结果的意义。
