Department of Physiology, University of Málaga, Málaga 29080, Spain.
Neuropharmacology. 2011 Jul-Aug;61(1-2):80-6. doi: 10.1016/j.neuropharm.2011.03.002. Epub 2011 Mar 17.
The aim of this study was to evaluate by quantitative receptor autoradiography the interactions between Neuropeptide Y Y1 (NPY Y1) and Galanin (GAL) receptors in the dorsal raphe nucleus (DRN) where both GAL receptors and NPY Y1 receptors exist. The ability of the GAL receptor antagonist M35 to block the GAL action was also evaluated. Double immunocytochemical staining of 5-hydroxytryptmine and c-Fos and stereology techniques were used to study the specific cell activation in the DRN after the intracerebroventricular coinjections of GAL and the NPY Y1/Y5 agonist [(125)I] Leu(31),Pro(34)PYY. GAL (0.3 nM) decreases [(125)I] Leu(31),Pro(34)PYY binding in the DRN by 48% (p < 0.01) as shown by quantitative receptor autoradiography. This effect was reversed with the GAL receptor antagonist M35. Intracerebroventricular coinjections of NPY Y1/Y5 agonist and GAL reduced the c-Fos expression in the serotoninergic cells induced by the NPY Y1/Y5 agonist in DRN. These results indicate the existence of antagonistic interactions between GAL receptors and NPY Y1 receptors in the DRN that may be of relevance in mood disorders.
本研究旨在通过定量受体放射自显影技术评估背缝核（DRN）中神经肽 Y Y1（NPY Y1）和甘丙肽（GAL）受体之间的相互作用，因为 DRN 中同时存在 GAL 受体和 NPY Y1 受体。还评估了 GAL 受体拮抗剂 M35 阻断 GAL 作用的能力。通过 5-羟色胺和 c-Fos 的双重免疫细胞化学染色和立体学技术，研究了 GAL 和 NPY Y1/Y5 激动剂 [(125)I] Leu(31),Pro(34)PYY 脑室内共注射后 DRN 中特定细胞的激活。GAL（0.3 nM）通过定量受体放射自显影显示，使 DRN 中 [(125)I] Leu(31),Pro(34)PYY 结合减少 48%（p < 0.01）。这种作用被 GAL 受体拮抗剂 M35 逆转。NPY Y1/Y5 激动剂和 GAL 的脑室内共注射减少了 NPY Y1/Y5 激动剂在 DRN 中诱导的 5-羟色胺能细胞中 c-Fos 的表达。这些结果表明，DRN 中 GAL 受体和 NPY Y1 受体之间存在拮抗相互作用，这可能与情绪障碍有关。
