废水中分离出的溶解性有机物对 17β-雌二醇结合的影响及其雌激素活性。
Influence of 17β-estradiol binding by dissolved organic matter isolated from wastewater effluent on estrogenic activity.
机构信息
Chemical Safety Division, National Academy of Agricultural Science, Seodundong, Gwonseongu, Suwon 441-707, Republic of Korea.
出版信息
Ecotoxicol Environ Saf. 2011 Jul;74(5):1280-7. doi: 10.1016/j.ecoenv.2011.02.010. Epub 2011 Mar 12.
The aims of this study were to determine the sorption coefficient (LogK(DOC)) of 17β-estradiol (E2), according to the size and composition of dissolved organic matter (DOM) isolated from wastewater effluent using a fluorescence quenching (FQ) method, and to measure the estrogenic changes due to the extent of E2 sorption onto effluent DOM (EfOM) by using an E-screen bioassay. The highest logK(DOC) of E2 for a DOM size fraction less than 0.2 μm was observed by 4.87 ± 1.87 Lkg(-1)(DOC), and its estrogenicity was the lowest among all the size fractions at 1.2 ng-EEQL⁻¹. However, E2 estrogenicity for a DOM size fraction less than 5 kDa was as high as that for the positive control due to the absence of fulvic acid- and protein-like DOM to bind with E2. The estrogenic activity for the hydrophobic fraction bound with E2 was significantly reduced to 1.6 ng-EEQL⁻¹, while that for the hydrophilic fraction having no binding with E2 was 2.6 ng-EEQL⁻¹, which was similar to that of the positive control (i.e., 2.8 ng-EEQL⁻¹). The results support a conclusion that the change in estrogenicity was due to the strong sorption affinity of E2 onto DOM.
本研究的目的是使用荧光猝灭（FQ）方法，根据从废水流出物中分离出的溶解性有机物（DOM）的大小和组成，确定 17β-雌二醇（E2）的分配系数（LogK(DOC)），并通过 E-screen 生物测定法测量由于 E2 对流出物 DOM（EfOM）的吸附程度而导致的雌激素变化。通过 4.87±1.87 Lkg-1(DOC)观察到小于 0.2 μm 的 DOM 大小分数中 E2 的最高 LogK(DOC)，并且在所有大小分数中其雌激素性最低，为 1.2 ng-EEQL-1。然而，由于没有富里酸和蛋白质样 DOM 与 E2 结合，小于 5 kDa 的 DOM 大小分数中的 E2 雌激素性与阳性对照一样高。与 E2 结合的疏水性部分的雌激素活性显著降低至 1.6 ng-EEQL-1，而与 E2 没有结合的亲水性部分的雌激素活性为 2.6 ng-EEQL-1，与阳性对照（即 2.8 ng-EEQL-1）相似。结果支持这样的结论，即雌激素活性的变化是由于 E2 对 DOM 的强吸附亲和力所致。
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