多发性硬化症中神经抗原特异性 CD8+和 CD4+T 细胞的克隆组成。
Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis.
机构信息
Department of Pathology, UT Southwestern Medical Center, 6000 Harry Hines Blvd., Dallas, TX 75390-9072, USA.
出版信息
J Neuroimmunol. 2011 May;234(1-2):131-40. doi: 10.1016/j.jneuroim.2011.02.001. Epub 2011 Mar 11.
Abstract
Patients with multiple sclerosis (MS) show a high prevalence of myelin-reactive CD8+ and CD4+ T-cell responses, which are the putative effectors/modulators of CNS neuropathology. Utilizing a novel combination of short-term culture, CFSE-based sorting and anchored PCR, we evaluated clonal compositions of neuroantigen-targeting T-cells from RRMS patients and controls. CDR3 region analysis of TCRβ chains revealed biased use of specific TCRBV-bearing CD4+ clones. CD8+ clones showed homology to published TCR from CNS-infiltrating T-cells in MS lesions. These studies are the first description of TCR usage of CNS-specific CD8+ T-cells and provide insights into their potential regulatory role in disease.
摘要
多发性硬化症（MS）患者表现出高频率的髓鞘反应性 CD8+ 和 CD4+ T 细胞反应，这些反应被认为是中枢神经系统神经病理学的效应物/调节剂。利用短期培养、CFSE 基于分选和锚定 PCR 的新组合，我们评估了 RRMS 患者和对照组中神经抗原靶向 T 细胞的克隆组成。TCRβ 链的 CDR3 区域分析显示特定 TCRBV 携带的 CD4+克隆的使用存在偏向性。CD8+克隆与 MS 病变中中枢神经系统浸润 T 细胞的已发表 TCR 具有同源性。这些研究首次描述了中枢神经系统特异性 CD8+T 细胞的 TCR 利用情况，并为其在疾病中的潜在调节作用提供了线索。
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