使用 2 种肺炎球菌共同蛋白实时聚合酶链反应检测方法对定植有肺炎链球菌的健康儿童进行检测。
Use of 2 pneumococcal common protein real-time polymerase chain reaction assays in healthy children colonized with Streptococcus pneumoniae.
机构信息
Centers for Diseases Control and Prevention, Atlanta, GA 30329, USA.
出版信息
Diagn Microbiol Infect Dis. 2011 Aug;70(4):452-4. doi: 10.1016/j.diagmicrobio.2010.09.006. Epub 2011 Mar 12.
Abstract
Polymerase chain reaction (PCR) offers promise in pneumococcal diagnostics, but whether nasopharyngeal carriage causes false-positive results in people colonized with Streptococcus pneumoniae is unknown. We found in serum no positive samples for pneumococcal DNA in 100 carriers and noncarriers using 2 different real-time PCR assays targeting lytA and psaA genes.
摘要
聚合酶链反应（PCR）在肺炎球菌诊断中具有应用前景，但鼻咽携带是否会导致定植有肺炎链球菌的人群出现假阳性结果尚不清楚。我们使用针对 lytA 和 psaA 基因的 2 种不同实时 PCR 检测方法，在 100 名携带和非携带鼻咽拭子者的血清中均未发现肺炎球菌 DNA 的阳性样本。
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