Yamagishi Junya, Wakaguri Hiroyuki, Sugano Sumio, Kawano Suguru, Fujisaki Kozo, Sugimoto Chihiro, Watanabe Junichi, Suzuki Yutaka, Kimata Isao, Xuan Xuenan
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
Parasitol Int. 2011 Jun;60(2):199-202. doi: 10.1016/j.parint.2011.03.001. Epub 2011 Mar 21.
A full-length cDNA library was constructed from the sporozoite of Cryptosporidium parvum. Normalized clones were subjected to Solexa shotgun sequencing, and then complete sequences for 1066 clones were reconfigured. Detailed analyses of the sequences revealed that 13.5% of the transcripts were spliced; the average and median 5' UTR lengths were 213.5 and 122 nucleotides, respectively. There were 148 inconsistencies out of 562 examined genes between the experimentally described cDNA sequence and the predicted sequence from its genome. In addition, we identified 118 sequences that had little homology against annotated genes of C. parvum as prospective candidates for addable genes. These observations should improve the reliability of C. parvum transcriptome and provide a versatile resource for further studies.
从小隐孢子虫的子孢子构建了全长cDNA文库。对标准化克隆进行了Solexa鸟枪法测序，然后对1066个克隆的完整序列进行了重新组装。对这些序列的详细分析表明，13.5%的转录本发生了剪接；5'UTR的平均长度和中位数长度分别为213.5个核苷酸和122个核苷酸。在所检测的562个基因中，实验描述的cDNA序列与其基因组预测序列之间存在148个不一致之处。此外，我们鉴定出118个与小隐孢子虫注释基因几乎没有同源性的序列，作为可添加基因的潜在候选序列。这些发现将提高小隐孢子虫转录组的可靠性，并为进一步研究提供丰富的资源。
