Key Laboratory of Animal Resistance Research, College of Life Science, Shandong Normal University, Ji'nan, China.
Acta Histochem. 2012 Feb;114(2):79-86. doi: 10.1016/j.acthis.2011.02.009. Epub 2011 Mar 12.
The ecotropic viral integration site-1 (Evi-1) is a transcription factor with two sets of zinc finger domains. It is an important regulator of the transforming growth factor beta superfamily. In the present study, we investigated the spatiotemporal expression patterns of Evi-1 using immunohistochemistry in ovaries from neonatal mice, gonadotropin-induced immature mice and mice in estrous cycle. Positive staining for Evi-1 was detected in the cytoplasm of oocytes. In postnatal mice, a high level of Evi-1 immunostaining was found from day 1 to 6, an intermediate level from day 10 to 16 and a low level on day 23. After gonadotropin treatment, Evi-1 was mainly expressed in small follicles and exhibited a very low level in large antral follicles. During the estrous cycle, the expression of Evi-1 was higher in diestrus and estrus than in proestrus and metestrus. Real-time PCR was performed to examine the relationship between Evi-1 mRNA and ovulation related genes (Ptgs2, Tnfaip6, Has2, Cd44, C1qbp). At 4 h after hCG treatment, Evi-1 mRNA was down-regulated whereas ovulation related genes were up-regulated. Overall, the results indicate that Evi-1 is expressed in a stage-specific manner during ovarian follicular development and may be involved in early follicle development.
嗜性逆转录病毒整合位点 1（Evi-1）是一种具有两组锌指结构域的转录因子。它是转化生长因子β超家族的重要调节因子。本研究采用免疫组织化学方法，研究了 Evi-1 在新生小鼠、促性腺激素诱导的未成熟小鼠和动情周期小鼠卵巢中的时空表达模式。Evi-1 在卵母细胞的细胞质中呈阳性染色。在新生小鼠中，从第 1 天到第 6 天，Evi-1 免疫染色水平较高，第 10 天到第 16 天为中等水平，第 23 天为低水平。在促性腺激素处理后，Evi-1 主要在小卵泡中表达，在大卵泡中表达水平非常低。在动情周期中，Evi-1 在动情期和发情期的表达高于发情前期和发情后期。实时 PCR 用于检测 Evi-1 mRNA 与排卵相关基因（Ptgs2、Tnfaip6、Has2、Cd44、C1qbp）之间的关系。在 hCG 处理后 4 小时，Evi-1 mRNA 下调，而排卵相关基因上调。总之，这些结果表明，Evi-1 在卵巢卵泡发育过程中以特定的阶段特异性方式表达，可能参与早期卵泡发育。
